Rabbit HDAC1 Polyclonal Antibody | anti-HDAC1 antibody

HDAC1 (C19) Antibody

Cat. Num. AAA440062

• Gene Names: HDAC1; HD1; RPD3; GON-10; RPD3L1
• Reactivity: Mouse, rat, human
• Applications: Western Blot
• Synonyms: HDAC1; Polyclonal Antibody; HDAC1 (C19) Antibody; anti-HDAC1 antibody

• Host: Rabbit
• Reactivity: Mouse, rat, human
• Clonality: Polyclonal
• Specificity: Mouse, rat, and human HDAC1
• Form/Format: 200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
• Concentration: 0.200 mg/ml (varies by lot)
• Sequence Length: 482

• Applicable Applications for anti-HDAC1 antibody: Western Blot (WB)
• Application Notes: Recommended western blotting starting dilution 1:200.
• Origin: HDAC1 (C19) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminal domain of human HDAC1.
• Immunogen: Synthetic peptide mapping to the carboxy terminal domain of human HDAC1.
• Preparation and Storage: Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Related Product Information for anti-HDAC1 antibody

HDAC1, the mammalian homologue of the yeast transcriptional regulator Rpd3p, was first purified using a trapoxin affinity matrix (1). Trapoxin is a cyclotetrapeptide that inhibits histone deacetylation in vivo and also correlates with an increase in the acetylation levels of all four core histones (1&2). These data support a role for HDAC1 as a key histone deacetylase. The function of HDAC1 has been further refined as responsible for the deacetylation of lysine residues on the N-terminal region of the core histones H2A, H2B, H3 and H4 (3&4).

References

1. Taunton J, Hassig CA, Schreiber SL. 1996. A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. Science 272:408-411.2. Hassig CA, Tong JK, Fleischer TC, Owa T, Grable PG, Ayer DE, Schreiber SL. 1998. A role for histone deacetylase activity in HDAC1-mediated transcriptional repression. PNAS 95(7):3519-3524.3. Lopez-Rodas G, Brosch G, Georgieva EI, Sendra R, Franco L, Loidl P. 1993. Histone deacetylase. A key enzyme for the binding of regulatory proteins to chromatin. FEBS lett 317(3):175-180.4. Johnson CA, White DA, Lavender JS, O?Neill LP, Turner BM. 2002. Human class I histone deacetylase complexes show enhanced catalytic activity in the presence of ATP and co-immunoprecipitated with ATP-dependent charperone protein Hsp-70.

NCBI and Uniprot Product Information

• NCBI GI #: 13128860
• NCBI GeneID: 3065
• NCBI Accession #: NP_004955.2
• NCBI GenBank Nucleotide #: NM_004964.2
• UniProt Accession #: Q13547; Q92534
• Molecular Weight: 55,103 Da
• NCBI Official Full Name: histone deacetylase 1
• NCBI Official Synonym Full Names: histone deacetylase 1
• NCBI Official Symbol: HDAC1
• NCBI Official Synonym Symbols: HD1; RPD3; GON-10; RPD3L1
• NCBI Protein Information: histone deacetylase 1; reduced potassium dependency, yeast homolog-like 1
• UniProt Protein Name: Histone deacetylase 1
• UniProt Gene Name: HDAC1
• UniProt Synonym Gene Names: RPD3L1; HD1
• UniProt Entry Name: HDAC1_HUMAN

NCBI Description

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.

Protein type: Hydrolase; Nuclear receptor co-regulator; EC 3.5.1.98

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: nucleoplasm; protein complex; Sin3 complex; nuclear chromatin; histone deacetylase complex; cytoplasm; NuRD complex; cytosol; nucleus; chromatin

Molecular Function: deacetylase activity; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); nucleosomal DNA binding; enzyme binding; transcription activator binding; NAD-dependent histone deacetylase activity (H3-K14 specific); histone deacetylase binding; protein deacetylase activity; protein N-terminus binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor activity; transcription factor binding

Biological Process: establishment and/or maintenance of chromatin architecture; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell cycle; protein amino acid deacetylation; negative regulation of gene expression, epigenetic; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; circadian regulation of gene expression; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; histone deacetylation; chromatin modification; odontogenesis of dentine-containing teeth; chromatin remodeling; ATP-dependent chromatin remodeling; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; gene expression; mitotic cell cycle; embryonic digit morphogenesis; blood coagulation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis