Rabbit anti-Human HCRTR1 Polyclonal Antibody | anti-HCRTR1 antibody

HCRTR1, ID (HCRTR1, Orexin receptor type 1, Hypocretin receptor type 1) (Biotin)

Cat. Num. AAA6305825

• Gene Names: HCRTR1; OX1R
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HCRTR1; Polyclonal Antibody; ID (HCRTR1; Orexin receptor type 1; Hypocretin receptor type 1) (Biotin); Hypocretin receptor type 1; anti-HCRTR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-HCRTR1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HCRTR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-298 amino acids from the Central region of human HCRTR1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HCRTR1 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HCRTR1 antibody detected the HCRTR1 protein (arrow).)

Related Product Information for anti-HCRTR1 antibody

The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B.

Product Categories/Family for anti-HCRTR1 antibody

Antibodies; Receptors

References

Peltonen, H.M., et al. Biochim. Biophys. Acta 1803(10):1206-1212(2010). Saus, E., et al. J Psychiatr Res 44(14):971-978(2010). Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010): Turunen, P.M., et al. Br. J. Pharmacol. 159(1):212-221(2010). El Firar, A., et al. FASEB J. 23(12):4069-4080(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 222080095
• NCBI GeneID: 3061
• NCBI Accession #: NP_001516.2
• NCBI GenBank Nucleotide #: NM_001525.2
• UniProt Accession #: O43613; Q9HBV6; A8K3A6
• Molecular Weight: 47,536 Da
• NCBI Official Full Name: orexin receptor type 1
• NCBI Official Synonym Full Names: hypocretin (orexin) receptor 1
• NCBI Official Symbol: HCRTR1
• NCBI Official Synonym Symbols: OX1R
• NCBI Protein Information: orexin receptor type 1; ox1-R; ox-1-R; orexin receptor 1; orexin receptor-1; hypocretin receptor 1; hypocretin receptor-1; hypocretin receptor type 1
• UniProt Protein Name: Orexin receptor type 1
• Protein Family: Orexin receptor
• UniProt Gene Name: HCRTR1
• UniProt Synonym Gene Names: Ox-1-R; Ox1-R; Ox1R
• UniProt Entry Name: OX1R_HUMAN

NCBI Description

The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. [provided by RefSeq, Jan 2009]

Uniprot Description

HCRTR1: Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p33

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; neuropeptide receptor activity; orexin receptor activity; peptide hormone binding; peptide binding

Biological Process: synaptic transmission; neuropeptide signaling pathway; feeding behavior; cellular response to hormone stimulus; regulation of circadian sleep/wake cycle, sleep