Rabbit HCRT Polyclonal Antibody | anti-HCRT antibody

HCRT Polyclonal Antibody

Cat. Num. AAA2563419

• Gene Names: HCRT; OX; PPOX; NRCLP1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, ELISA
• Purity: Antigen Affinity Purification
• Synonyms: HCRT; Polyclonal Antibody; HCRT Polyclonal Antibody; HCRT1; HCRT2; Hypocretin (orexin) neuropeptide precursor; Hypocretin 1; Hypocretin 2; Hypocretin; NRCLP1; Orexin; Orexin A; Orexin B; Orexin precursor; OX; PPORX; PPOX; PREPROOREXIN; anti-HCRT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Form/Format: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
• Concentration: 0.6mg/mL (varies by lot)
• Sequence Length: 131

• Applicable Applications for anti-HCRT antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:25-1:100; ELISA: 1:2000-1:5000
• Immunogen: Synthetic peptide of human HCRT
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using HCRT Polyclonal Antibody at dilution of 1:25(×200))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human tonsil tissue using HCRT Polyclonal Antibody at dilution of 1:25(×200))

Related Product Information for anti-HCRT antibody

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.

Product Categories/Family for anti-HCRT antibody

Cancer; Metabolism; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 4557635
• NCBI GeneID: 3060
• NCBI Accession #: NP_001515.1
• NCBI GenBank Nucleotide #: NP_001515.1
• UniProt Accession #: O43612
• NCBI Official Full Name: orexin
• NCBI Official Synonym Full Names: hypocretin neuropeptide precursor
• NCBI Official Symbol: HCRT
• NCBI Official Synonym Symbols: OX; PPOX; NRCLP1
• NCBI Protein Information: orexin
• UniProt Protein Name: Orexin
• Protein Family: Orexin
• UniProt Gene Name: HCRT
• UniProt Synonym Gene Names: OX; PPORX; PPOX; Hcrt; Hcrt1; Hcrt2
• UniProt Entry Name: OREX_HUMAN

NCBI Description

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq, Jan 2010]

Uniprot Description

HCRT: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. Defects in HCRT are the cause of narcolepsy type 1 (NRCLP1). Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed. Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. Belongs to the orexin family.

Protein type: Hormone

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: synaptic vesicle; rough endoplasmic reticulum; perinuclear region of cytoplasm; extracellular region; cell junction; secretory granule

Molecular Function: type 2 hypocretin receptor binding; type 1 hypocretin receptor binding

Biological Process: eating behavior; negative regulation of potassium ion transport; regulation of neurotransmitter secretion; synaptic transmission; elevation of cytosolic calcium ion concentration; negative regulation of transmission of nerve impulse; neuropeptide signaling pathway; negative regulation of DNA replication; protein kinase C activation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of calcium ion transport; regulation of excitatory postsynaptic membrane potential; positive regulation of transmission of nerve impulse

Disease: Narcolepsy 1