Rabbit anti-Human Hck Polyclonal Antibody | anti-HCK antibody

Hck, NT (Hck, Tyrosine-protein kinase HCK, B-cell/myeloid kinase, Hematopoietic cell kinase, Hemopoietic cell kinase, p56-HCK/p59-HCK)

Cat. Num. AAA646538

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Hck; Polyclonal Antibody; NT (Hck; Tyrosine-protein kinase HCK; B-cell/myeloid kinase; Hematopoietic cell kinase; Hemopoietic cell kinase; p56-HCK/p59-HCK); Anti -Hck; anti-HCK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-HCK antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: Mouse Hck antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-101 amino acids from the N-terminal region of Mouse Hck.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Hck Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the Hck antibody detected the Hck protein (arrow).)

Related Product Information for anti-HCK antibody

The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may play a role in the innate immune response and the STAT5 signaling pathway. Alternative translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.

Product Categories/Family for anti-HCK antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 14627116
• UniProt Accession #: Q95M30
• Molecular Weight: 57,096 Da
• NCBI Official Full Name: hck protein
• UniProt Protein Name: Tyrosine-protein kinase HCK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: HCK
• UniProt Entry Name: HCK_MACFA

Uniprot Description

Function: Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS

By similarity.

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulation: Subject to autoinhibition, mediated by intramolecular interactions involving the SH2 and SH3 domains. Kinase activity is also regulated by phosphorylation at regulatory tyrosine residues. Phosphorylation at Tyr-389 is required for optimal activity. Phosphorylation at Tyr-500 inhibits kinase activity

By similarity.

Subunit structure: Interacts with ADAM15. Interacts with FASLG. Interacts with ARRB1 and ARRB2. Interacts with FCGR1A; the interaction may be indirect. Interacts with IL6ST. Interacts (via SH3 domain) with ELMO1. Interacts (via SH3 domain) with TP73. Interacts with YAP1. Interacts with ABL1 and ITGB1, and thereby recruits ABL1 to activated ITGB1. Interacts (via SH2 domain) with FLT3 (tyrosine phosphorylated). Interacts with CBL. Interacts with VAV1, WAS and RAPGEF1

By similarity.

Subcellular location: Cytoplasmic vesicle › secretory vesicle

By similarity. Cytoplasm › cytosol

By similarity. Cell membrane; Lipid-anchor. Membrane › caveola; Lipid-anchor

By similarity. Cell junction › focal adhesion

By similarity. Cytoplasm › cytoskeleton

By similarity. Golgi apparatus

By similarity. Cytoplasmic vesicle

By similarity. Lysosome

By similarity. Nucleus

By similarity. Note: A small fraction is associated with caveolae. Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3. Colocalizes with the actin cytoskeleton at focal adhesions

By similarity.

Post-translational modification: Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation at Tyr-389 increases kinase activity. Phosphorylation at Tyr-500 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-500, suggesting that this site may be a target of other kinases

By similarity.Ubiquitinated by CBL, leading to its degradation via the proteasome

By similarity.Palmitoylation requires prior myristoylation. Palmitoylation is required for caveolar localization

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.Contains 1 protein kinase domain.Contains 1 SH2 domain.Contains 1 SH3 domain.