Western Blot (WB)
(HCK Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HCK antibody detected the HCK protein (arrow).)
Rabbit HCK Polyclonal Antibody | anti-HCK antibody
HCK Antibody (N-term)
Western Blot (WB)
(HCK Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HCK antibody detected the HCK protein (arrow).)
Western Blot (WB)
(HCK Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HCK antibody detected the HCK protein (arrow).)
Immunohistochemistry (IHC)
(HCK Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HCK Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(HCK Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HCK Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(HCK Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(HCK Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
family of tyrosine kinases. This protein is primarily hemopoietic,
particularly in cells of the myeloid and B-lymphoid lineages. It
may help couple the Fc receptor to the activation of the
respiratory burst. In addition, it may play a role in neutrophil
migration and in the degranulation of neutrophils. Multiple
isoforms with different subcellular distributions are produced due
to both alternative splicing and the use of alternative translation
initiation codons, including a non-AUG (CUG) codon. [provided by
RefSeq].
Kennah, E., et al. Blood 113(19):4646-4655(2009)
Voss, M., et al. BMC Immunol. 10, 53 (2009) :
Rikova, K., et al. Cell 131(6):1190-1203(2007)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010]
Uniprot Description
Hck: a protein-tyrosine kinase of the Src family that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms that have different subcellular localization.
Protein type: Kinase, protein; Oncoprotein; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; EC 2.7.10.2; TK group; Src family
Chromosomal Location of Human Ortholog: 20q11-q12
Cellular Component: Golgi apparatus; extrinsic to internal side of plasma membrane; transport vesicle; cell projection; focal adhesion; lysosome; actin filament; caveola; nucleus; cytosol
Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding
Biological Process: integrin-mediated signaling pathway; respiratory burst after phagocytosis; peptidyl-tyrosine phosphorylation; viral reproduction; cytokine and chemokine mediated signaling pathway; protein amino acid autophosphorylation; regulation of phagocytosis; protein amino acid phosphorylation; regulation of defense response to virus by virus; regulation of cell shape; leukocyte migration during immune response; positive regulation of actin filament polymerization; regulation of transcription factor activity; leukocyte degranulation; regulation of inflammatory response; positive regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; innate immune response; mesoderm development; cell adhesion; cell differentiation; inflammatory response; innate immune response-activating signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis
Research Articles on HCK
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Product Notes
The HCK hck (Catalog #AAA9206253) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 131-156. The HCK Antibody (N-term) reacts with Human (Predicted Reactivity: Monkey, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's HCK can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the HCK hck for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HCK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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