Rabbit anti-Human, Mouse HBA2 Polyclonal Antibody | anti-HBA2 antibody

HBA2, ID (HBA1, Hemoglobin subunit alpha, Alpha-globin, Hemoglobin alpha chain) (MaxLight 650)

Cat. Num. AAA6305578

• Gene Names: HBA2; HBH; HBA-T2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: HBA2; Polyclonal Antibody; ID (HBA1; Hemoglobin subunit alpha; Alpha-globin; Hemoglobin alpha chain) (MaxLight 650); HBA1; Hemoglobin alpha chain; anti-HBA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HBA2. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-HBA2 antibody: Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 100-128 in the central region of human HBA2. Species sequence homology: monkey
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse liver tissue lysates (35ug/lane) usingMBS6001950. HBA2 (arrow) was detected usingMBS6001950.)

Western Blot (WB)

(Western Blot analysis of human fetal heart lysate (20ug) using MBS6001950 (1:2000). Secondary goat anti-rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 15kD)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human lung carcinoma usingMBS6001950, followed by peroxidase-conjugated to the secondary antibody, and DAB staining. This data demonstrates the use of MBS6001950 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis: Right histogram: K562 cells using MBS6001950 Left histogram: Isotype negative control cell. FITC-conjugated goat anti-rabbit IgG was used as secondary antibody.)

Product Categories/Family for anti-HBA2 antibody

Antibodies; Hemoglobin

References

1. Sessa, R., et al. Am. J. Hematol. 85(2):143-144(2010). 2. Sharma, V., et al. Hematology 14(5):297-300(2009). 3. Waye, J.S., et al. Hemoglobin 33(6):519-522(2009). 4. Roy, P., et al. Hemoglobin 33(6):486-491(2009). 5. Joly, P., et al. Hemoglobin 33(3):196-205(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 57013850
• NCBI GeneID: 3040
• UniProt Accession #: P69905; P01922; Q1HDT5; Q3MIF5; Q53F97; Q96KF1; Q9NYR7; Q9UCM0
• Molecular Weight: 15,258 Da
• NCBI Official Full Name: Hemoglobin subunit alpha
• NCBI Official Synonym Full Names: hemoglobin, alpha 2
• NCBI Official Symbol: HBA2
• NCBI Official Synonym Symbols: HBH; HBA-T2
• NCBI Protein Information: hemoglobin subunit alpha; alpha globin; alpha-globin; alpha-2 globin; hemoglobin alpha chain
• UniProt Protein Name: Hemoglobin subunit alpha
• UniProt Gene Name: HBA1
• UniProt Entry Name: HBA_HUMAN

NCBI Description

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

HBA1: Involved in oxygen transport from the lung to the various peripheral tissues. Defects in HBA1 may be a cause of Heinz body anemias (HEIBAN). This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency. Defects in HBA1 are the cause of alpha-thalassemia (A- THAL). The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non- deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers. Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non- immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders. Defects in HBA1 are the cause of hemoglobin H disease (HBH). HBH is a form of alpha-thalassemia due to the loss of three alpha genes. This results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia. Untreated, most patients die in childhood or early adolescence. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: membrane; hemoglobin complex; extracellular region; cytosol

Molecular Function: haptoglobin binding; protein binding; peroxidase activity; iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; oxygen transport; bicarbonate transport; protein heterooligomerization; hydrogen peroxide catabolic process

Disease: Hemoglobin H Disease; Heinz Body Anemias; Alpha-thalassemia