Rabbit HAT1 Polyclonal Antibody | anti-HAT1 antibody

HAT1 polyclonal antibody

Cat. Num. AAA3014664

• Gene Names: HAT1; KAT1
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: HAT1; Polyclonal Antibody; HAT1 polyclonal antibody; Histone acetyltransferase 1; anti-HAT1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: HAT1 polyclonal antibody detects endogenous levels of HAT1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-HAT1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000
• Immunogen: Synthetic peptide, corresponding to Human HAT1
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of HAT1 polyclonal antibody at 1:500 dilutionLane1:EC9706 whole cell lysate(40ug)Lane2: SGC7901 whole cell lysate(40ug)Lane3: HCT116 whole cell lysate(40ug)Lane4: CT-26 whole cell lysate(40ug)Lane5: PC12 whole cell lysate(40ug))

Related Product Information for anti-HAT1 antibody

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones.Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6 have been identified as histone deacetylases.

NCBI and Uniprot Product Information

• NCBI GI #: 4504341
• NCBI GeneID: 8520
• NCBI Accession #: NP_003633.1
• NCBI GenBank Nucleotide #: NM_003642.3
• UniProt Accession #: O14929; Q49A44; Q53QF0; Q53SU4; Q6P594; Q8WWB9
• Molecular Weight: 49,513 Da
• NCBI Official Full Name: histone acetyltransferase type B catalytic subunit
• NCBI Official Synonym Full Names: histone acetyltransferase 1
• NCBI Official Symbol: HAT1
• NCBI Official Synonym Symbols: KAT1
• NCBI Protein Information: histone acetyltransferase type B catalytic subunit
• UniProt Protein Name: Histone acetyltransferase type B catalytic subunit
• Protein Family: HAT1-interacting factor
• UniProt Gene Name: HAT1
• UniProt Synonym Gene Names: KAT1
• UniProt Entry Name: HAT1_HUMAN

NCBI Description

The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

HAT1: Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage. Belongs to the HAT1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.48; Acetyltransferase

Chromosomal Location of Human Ortholog: 2q31.2-q33.1

Cellular Component: nucleoplasm; nuclear matrix; protein complex; intracellular membrane-bound organelle; cytoplasm; nuclear chromatin; nucleus

Molecular Function: H4 histone acetyltransferase activity; protein binding; histone acetyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; DNA replication-independent nucleosome assembly; internal protein amino acid acetylation; DNA replication-dependent nucleosome assembly; DNA packaging; response to nutrient; chromatin silencing at telomere