Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with HAND2 Antibody (Center).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HAND2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).HAND2 immunoreactivity is localized to Nucleus significantly.)
Rabbit anti-Human HAND2 Polyclonal Antibody | anti-HAND2 antibody
HAND2, ID (HAND2, BHLHA26, DHAND, Heart-and neural crest derivatives-expressed protein 2, Class A basic helix-loop-helix protein 26, Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2) (Biotin)
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with HAND2 Antibody (Center).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HAND2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).HAND2 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with HAND2 Antibody (Center).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HAND2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).HAND2 immunoreactivity is localized to Nucleus significantly.)
Western Blot (WB)
(HAND2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HAND2 antibody detected the HAND2 protein (arrow).)
Western Blot (WB)
(HAND2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HAND2 antibody detected the HAND2 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development. [provided by RefSeq, Jul 2008]
Uniprot Description
HAND2: Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3'.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 4q33
Cellular Component: transcription factor complex; protein complex; nuclear chromatin
Molecular Function: protein binding; protein homodimerization activity; transcription activator binding; AT DNA binding; protein heterodimerization activity; transcription coactivator activity; transcription factor binding
Biological Process: adult heart development; heart morphogenesis; tongue development; neural crest cell development; thymus development; transcription, DNA-dependent; in utero embryonic development; apoptosis; negative regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; heart development; mesenchymal cell proliferation; palate development; negative regulation of DNA binding; odontogenesis of dentine-containing teeth; peripheral nervous system neuron development; sympathetic nervous system development; regulation of transcription, DNA-dependent; suckling behavior; positive regulation of transcription from RNA polymerase II promoter; negative regulation of osteoblast differentiation; angiogenesis; heart looping; embryonic digit morphogenesis
Research Articles on HAND2
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Product Notes
The HAND2 hand2 (Catalog #AAA6305385) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HAND2, ID (HAND2, BHLHA26, DHAND, Heart-and neural crest derivatives-expressed protein 2, Class A basic helix-loop-helix protein 26, Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HAND2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HAND2 hand2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HAND2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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