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Western Blot (WB) (Western blot analysis of anti-ABAD Pab in mouse kidney tissue lysate.ABAD(arrow) was detected using the purified Pab.)

Rabbit anti-Human HADH2 Polyclonal Antibody | anti-HADH2 antibody

HADH2, CT (Hydroxyacyl-Coenzyme A Dehydrogenase Type II, AB-binding Alcohol Dehydrogenase, ABAD, CAMR, DUPXp11.22, ERAB, HCD2, HSD17B10, MHBD, Mitochondrial Ribonuclease P Protein 2, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, Type II HADH, Type 10 17b-HS

Gene Names
HSD17B10; ABAD; CAMR; ERAB; HCD2; MHBD; HADH2; MRPP2; MRX17; MRX31; SCHAD; MRXS10; SDR5C1; HSD10MD; 17b-HSD10; DUPXp11.22
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
HADH2; Polyclonal Antibody; CT (Hydroxyacyl-Coenzyme A Dehydrogenase Type II; AB-binding Alcohol Dehydrogenase; ABAD; CAMR; DUPXp11.22; ERAB; HCD2; HSD17B10; MHBD; Mitochondrial Ribonuclease P Protein 2; MRPP2; MRX17; MRX31; MRXS10; SCHAD; SDR5C1; Type II HADH; Type 10 17b-HS; 3-hydroxyacyl-CoA Dehydrogenase Type 2; 3-hydroxyacyl-CoA Dehydrogenase Type II; 3-hydroxy-2-methylbutyryl-CoA Dehydrogenase; 17beta-hydroxysteroid Dehydrogenase 10; EC=1.1.1.35; EC=1.1.1.178; Endoplasmic Reticulum-associated Amyloid beta Peptide Binding Protein; Hydroxyacyl CoA Dehydrogenase Type II; Hydroxysteroid (17-beta) Dehydrogenase 10; Mitochondrial RNase P Protein 2; Short Chain Dehydrogenase/reductase Family 5C Member 1; Short-chain Type Dehydrogenase/reductase XH98G2; anti-HADH2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ABAD.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-HADH2 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human ABAD (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ABAD Pab in mouse kidney tissue lysate.ABAD(arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-ABAD Pab in mouse kidney tissue lysate.ABAD(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry on Formalin-fixed and paraffin-embedded human prostate tissue. (Top)Coloration with the Anti-ABAD antibody at a 1:20 concentration. (Bottom)Antibody adsorbed to the antigen (peptide), negative control.Disappearance of cytoplasmic staining indicating the antibody is specific. Data and protocol courtesy of Marie-Helène Levesque, Centre de Recherche du CHUL, Canada.)

Immunohistochemistry (IHC) (Immunohistochemistry on Formalin-fixed and paraffin-embedded human prostate tissue. (Top)Coloration with the Anti-ABAD antibody at a 1:20 concentration. (Bottom)Antibody adsorbed to the antigen (peptide), negative control.Disappearance of cytoplasmic staining indicating the antibody is specific. Data and protocol courtesy of Marie-Helène Levesque, Centre de Recherche du CHUL, Canada.)

Western Blot (WB)

(ABAD Antibody (C214) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ABAD antibody detected the ABAD protein (arrow).)

Western Blot (WB) (ABAD Antibody (C214) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ABAD antibody detected the ABAD protein (arrow).)
Product Categories/Family for anti-HADH2 antibody
References
1. FASEB J. 19(6), 597-598 (2005). 2. J. Mol. Biol. 342(3), 943-952 (2004). 3. Science 304(5669), 448-452 (2004). 4. FEBS Lett. 451(3), 238-242 (1999). 5. J. Biol. Chem. 274(21), 15014-15019 (1999).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28.1 kDa (271aa), confirmed by MALDI-TOF.
NCBI Official Full Name
3-hydroxyacyl-CoA dehydrogenase type-2 isoform 1
NCBI Official Synonym Full Names
hydroxysteroid 17-beta dehydrogenase 10
NCBI Official Symbol
HSD17B10
NCBI Official Synonym Symbols
ABAD; CAMR; ERAB; HCD2; MHBD; HADH2; MRPP2; MRX17; MRX31; SCHAD; MRXS10; SDR5C1; HSD10MD; 17b-HSD10; DUPXp11.22
NCBI Protein Information
3-hydroxyacyl-CoA dehydrogenase type-2
UniProt Protein Name
3-hydroxyacyl-CoA dehydrogenase type-2
UniProt Gene Name
HADH2
UniProt Synonym Gene Names
HSD17B10
UniProt Entry Name
Q6IBS9_HUMAN

NCBI Description

This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Aug 2014]

Uniprot Description

Sequence similarities: Belongs to the short-chain dehydrogenases/reductases (SDR) family. RuleBase RU000363

Research Articles on HADH2

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Product Notes

The HADH2 hadh2 (Catalog #AAA6479375) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HADH2, CT (Hydroxyacyl-Coenzyme A Dehydrogenase Type II, AB-binding Alcohol Dehydrogenase, ABAD, CAMR, DUPXp11.22, ERAB, HCD2, HSD17B10, MHBD, Mitochondrial Ribonuclease P Protein 2, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, Type II HADH, Type 10 17b-HS reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HADH2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HADH2 hadh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HADH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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