Rabbit anti-Human, Mouse HACL1 Polyclonal Antibody | anti-HACL1 antibody

HACL1 (2-Hydroxyacyl-CoA Lyase 1,2-Hydroxyphytanoyl-CoA Lyase, 2-HPCL, HPCL2, HPCL, HSPC279, FLJ55041, Phytanoyl-CoA 2-hydroxylase 2, PHYH2) (FITC)

Cat. Num. AAA6479521

• Gene Names: HACL1; HPCL; HPCL2; PHYH2; 2-HPCL
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: HACL1; Polyclonal Antibody; HACL1 (2-Hydroxyacyl-CoA Lyase 1; 2-Hydroxyphytanoyl-CoA Lyase; 2-HPCL; HPCL2; HPCL; HSPC279; FLJ55041; Phytanoyl-CoA 2-hydroxylase 2; PHYH2) (FITC); EC=4.1.-.-; anti-HACL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PHYH2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HACL1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PHYH2 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PHYH2 Antibody (C-term) in mouse liver tissue lysates (35ug/lane). PHYH2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PHYH2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-HACL1 antibody

Antibodies; Enzymes

References

1. Kikuchi, M., et.al., J. Biol. Chem. 279(1), 421-428 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 93004078
• NCBI GeneID: 26061
• NCBI Accession #: NP_036392.2
• NCBI GenBank Nucleotide #: NM_012260.3
• UniProt Accession #: Q9UJ83; Q9BV42; Q9P0A2; B4DWI1; B4DXI5; E9PEN4
• Molecular Weight: 54,724 Da
• NCBI Official Full Name: 2-hydroxyacyl-CoA lyase 1 isoform a
• NCBI Official Synonym Full Names: 2-hydroxyacyl-CoA lyase 1
• NCBI Official Symbol: HACL1
• NCBI Official Synonym Symbols: HPCL; HPCL2; PHYH2; 2-HPCL
• NCBI Protein Information: 2-hydroxyacyl-CoA lyase 1; 1600020H07Rik; 2-hydroxyphytanol-CoA lyase; phytanoyl-CoA hydroxylase 2; 2-hydroxyphytanoyl-CoA lyase; phytanoyl-CoA 2-hydroxylase 2
• UniProt Protein Name: 2-hydroxyacyl-CoA lyase 1
• Protein Family: 2-hydroxyacyl-CoA lyase
• UniProt Gene Name: HACL1
• UniProt Synonym Gene Names: HPCL; HPCL2; PHYH2; 2-HPCL
• UniProt Entry Name: HACL1_HUMAN

Uniprot Description

HACL1: Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde. Belongs to the TPP enzyme family.

Protein type: EC 4.1.-.-; Lyase

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: peroxisomal matrix; peroxisome

Molecular Function: carbon-carbon lyase activity; identical protein binding; magnesium ion binding; thiamin pyrophosphate binding; cofactor binding; receptor binding

Biological Process: cellular lipid metabolic process; protein oligomerization; fatty acid alpha-oxidation