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Western Blot (WB) (Western Blot (WB) analysis of H6PD polyclonal antibody)

Rabbit H6PD Polyclonal Antibody | anti-H6PD antibody

H6PD Polyclonal Antibody

Gene Names
H6PD; GDH; G6PDH; H6PDH; CORTRD1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
H6PD; Polyclonal Antibody; H6PD Polyclonal Antibody; GDH/6PGL endoplasmic bifunctional protein; GDH; anti-H6PD antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
H6PD polyclonal antibody detects endogenous levels of H6PD protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
791
Applicable Applications for anti-H6PD antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:10-1:100
Immunogen
Recombinant full length Human H6PD.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of H6PD polyclonal antibody)

Western Blot (WB) (Western Blot (WB) analysis of H6PD polyclonal antibody)
Related Product Information for anti-H6PD antibody
H6PD (hexose-6-phosphate dehydrogenase, GDH/6PGL endoplasmic bifunctional protein) is a 789 amino acid protein encoded by the human gene H6PD. The N-terminal section of H6PD belongs to the glucose-6-phosphate dehydrogenase family, while the C-terminal section belongs to the glucosamine/galactosamine-6-phosphate isomerase family, 6-phosphogluconolactonase subfamily. H6PD is responsible primarily for the oxidation of glucose-6-phosphate and glucose. It also oxidizes other hexose-6-phosphates. H6PD catalyzes the conversion of glucose 6-phosphate to 6-phosphogluconolactone within the lumen of the endoplasmic reticulum, thereby generating reduced nicotinamide adenine dinucleotide phosphate. Reduced nicotinamide adenine dinucleotide phosphate is a necessary cofactor for the reductase activity of 11beta-hydroxysteroid dehydrogenase type 1, which converts hormonally inactive cortisone to active cortisol (in rodents, 11-dehydrocorticosterone to corticosterone).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 89kDa
NCBI Official Full Name
GDH/6PGL endoplasmic bifunctional protein isoform 2
NCBI Official Synonym Full Names
hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase
NCBI Official Symbol
H6PD
NCBI Official Synonym Symbols
GDH; G6PDH; H6PDH; CORTRD1
NCBI Protein Information
GDH/6PGL endoplasmic bifunctional protein
UniProt Protein Name
GDH/6PGL endoplasmic bifunctional protein
UniProt Gene Name
H6PD
UniProt Synonym Gene Names
GDH; 6PGL
UniProt Entry Name
G6PE_HUMAN

NCBI Description

There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells. [provided by RefSeq, Jul 2008]

Research Articles on H6PD

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Product Notes

The H6PD h6pd (Catalog #AAA3011574) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The H6PD Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's H6PD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:10-1:100. Researchers should empirically determine the suitability of the H6PD h6pd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H6PD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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