Immunofluorescence (IF)
(NIH3T3 cells were stained with the antibody and with DAPI. The cells were immunofluorescently labelled with the antibody (left) followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shownon the right)
Rabbit anti-Human H3K9ac Polyclonal Antibody | anti-H3K9ac antibody
H3K9ac polyclonal antibody
Note: This information is only intended as a guide. The optimal dilutions must be determined by the user.
Immunofluorescence (IF)
(NIH3T3 cells were stained with the antibody and with DAPI. The cells were immunofluorescently labelled with the antibody (left) followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shownon the right)
Immunofluorescence (IF)
(NIH3T3 cells were stained with the antibody and with DAPI. The cells were immunofluorescently labelled with the antibody (left) followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shownon the right)
Testing Data
Testing Data
Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
NCBI and Uniprot Product Information
NCBI Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
Uniprot Description
H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 6p22.2
Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus
Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity
Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; DNA methylation on cytosine; regulation of gene expression, epigenetic; DNA replication-dependent nucleosome assembly
Research Articles on H3K9ac
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Product Notes
The H3K9ac hist1h3a (Catalog #AAA841016) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The H3K9ac polyclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's H3K9ac can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ChIP, ELISA (EIA), Dot Blot (DB). WB: 1:1000, Dot Blot: 1:20,000, ChIP: 1-2 ul/ChIP, IF: 1:500, ELISA: 1:1000. Note: This information is only intended as a guide. The optimal dilutions must be determined by the user. Researchers should empirically determine the suitability of the H3K9ac hist1h3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H3K9ac, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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