Rabbit H-NUC Polyclonal Antibody | anti-H-NUC antibody

H-NUC Antibody

Cat. Num. AAA9405062

• Gene Names: CDC27; APC3; HNUC; NUC2; H-NUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: H-NUC; Polyclonal Antibody; H-NUC Antibody; CDC27Hs; Cell division cycle protein 27 homolog; anti-H-NUC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total H-NUC protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 830

• Applicable Applications for anti-H-NUC antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:3000
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human H-NUC.
• Target Name: H-NUC
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from HUVEC cells, HepG2 cells and COLO205 cells, using H-NUC antibody.)

Related Product Information for anti-H-NUC antibody

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.Tugendreich S., Proc. Natl. Acad. Sci. U.S.A. 90:10031-10035(1993).Chen P.L., Cell Growth Differ. 6:199-210(1995).Kraft C., EMBO J. 22:6598-6609(2003).

Product Categories/Family for anti-H-NUC antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 167466177
• NCBI GeneID: 996
• NCBI Accession #: NP_001107563.1
• NCBI GenBank Nucleotide #: NM_001114091.2
• UniProt Accession #: P30260; Q16349; Q96F35; G3V1C4
• Molecular Weight: 92,612 Da
• NCBI Official Full Name: cell division cycle protein 27 homolog isoform 1
• NCBI Official Synonym Full Names: cell division cycle 27
• NCBI Official Symbol: CDC27
• NCBI Official Synonym Symbols: APC3; HNUC; NUC2; H-NUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E
• NCBI Protein Information: cell division cycle protein 27 homolog
• UniProt Protein Name: Cell division cycle protein 27 homolog
• UniProt Gene Name: CDC27
• UniProt Synonym Gene Names: ANAPC3; D0S1430E; D17S978E; APC3; CDC27Hs
• UniProt Entry Name: CDC27_HUMAN

NCBI Description

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]

Uniprot Description

CDC27: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C is composed of at least 12 subunits. Interacts with RB. Interacts with FAM168B/MANI. Belongs to the APC3/CDC27 family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleoplasm; centrosome; anaphase-promoting complex; spindle microtubule; cytoplasm; spindle; nucleus; cytosol

Molecular Function: protein binding; protein phosphatase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell proliferation; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; mitotic metaphase/anaphase transition