Typical Testing Data/Standard Curve (for reference only)
Caspase-1 ELISA Kit | Casp1 elisa kit
Caspase-1 (Mouse) ELISA Kit
Gene Names
Casp1; Ice; Il1bc
Synonyms
Caspase-1; Caspase-1 (Mouse) ELISA Kit; Casp1 elisa kit
Specificity
The Candida albicans IgA, IgG and IgM ELISA tests were verified in an internal study. The sensitivity and specificity in both cases exceeded 92%
Sequence Length
402
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Casp1 elisa kit
Intended Uses: The Candida albicans IgA, IgG and IgM ELISA tests are qualitative immunoassays for the demonstration of human IgA, IgG and IgM antibodies directed against Candida albicans. The evaluation of individual immunoglobulin classes can be used for the determination of pathogen contact.
Principle of the Assay: The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in the field of infectious serology. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate p-nitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically!!Background/Introduction: Candida spp. are ubiquitous yeast-like fungi, which can even be demonstrated in around 75 % of all healthy individuals in the form of skin and mucous membrane colonization. They live as harmless saprophytes in the throat, trachea, stomach, and large and small intestines. The microorganisms are normally not capable of breaking the defence mechanisms of the human skin and mucous membranes and successfully challenging the immune system, but numerous risk factors may, however, significantly increase the likelihood of Candida albicans or related fungi becoming established, resulting in mycosis. Candida albicans is the primary cause of candidiasis. Yeasts of the genus Candida frequently cause infections of the mucous membranes, e. g. oral thrush, vaginal mycosis, or nappy-rash, but can also be responsible for systemic organ mycosis affecting the eyes, liver, kidneys or lungs and also be the cause of Candida septicaemia. Apart from the yeast form, which primarily causes superficial infections, so called pseudo mycelia are a further morphologic manifestation of the pathogen. Germ tubes and the development of pseudo mycelia mainly occur in cases of systemic mycosis. Candida spp. produce and excrete a range of destructive enzymes, that enable the facultative pathogenic microorganisms to penetrate mucous membrane barriers and blood vessels. In general, Candida spp. are transmitted from person to person by smear contamination. The primary portal of entry is the oral cavity. Changes in the fungistatic properties of the skin, which are a consequence of a slightly acidic pH value and the antagonistic bacterial flora, can facilitate the establishment of superficial candidiasis of the skin surface. Systemic mycosis results from colonization of mucous membranes, particularly in the gastrointestinal tract.
Principle of the Assay: The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in the field of infectious serology. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate p-nitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically!!Background/Introduction: Candida spp. are ubiquitous yeast-like fungi, which can even be demonstrated in around 75 % of all healthy individuals in the form of skin and mucous membrane colonization. They live as harmless saprophytes in the throat, trachea, stomach, and large and small intestines. The microorganisms are normally not capable of breaking the defence mechanisms of the human skin and mucous membranes and successfully challenging the immune system, but numerous risk factors may, however, significantly increase the likelihood of Candida albicans or related fungi becoming established, resulting in mycosis. Candida albicans is the primary cause of candidiasis. Yeasts of the genus Candida frequently cause infections of the mucous membranes, e. g. oral thrush, vaginal mycosis, or nappy-rash, but can also be responsible for systemic organ mycosis affecting the eyes, liver, kidneys or lungs and also be the cause of Candida septicaemia. Apart from the yeast form, which primarily causes superficial infections, so called pseudo mycelia are a further morphologic manifestation of the pathogen. Germ tubes and the development of pseudo mycelia mainly occur in cases of systemic mycosis. Candida spp. produce and excrete a range of destructive enzymes, that enable the facultative pathogenic microorganisms to penetrate mucous membrane barriers and blood vessels. In general, Candida spp. are transmitted from person to person by smear contamination. The primary portal of entry is the oral cavity. Changes in the fungistatic properties of the skin, which are a consequence of a slightly acidic pH value and the antagonistic bacterial flora, can facilitate the establishment of superficial candidiasis of the skin surface. Systemic mycosis results from colonization of mucous membranes, particularly in the gastrointestinal tract.
Product Categories/Family for Casp1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,576 Da
NCBI Official Full Name
caspase-1
NCBI Official Synonym Full Names
caspase 1
NCBI Official Symbol
Casp1
NCBI Official Synonym Symbols
Ice; Il1bc
NCBI Protein Information
caspase-1
UniProt Protein Name
Caspase-1
Protein Family
UniProt Gene Name
Casp1
UniProt Synonym Gene Names
Il1bc; Il1bce; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme
UniProt Entry Name
CASP1_RAT
NCBI Description
apoptotic protease that may play a role in inflammation and apoptosis in male sex organs [RGD, Feb 2006]
Uniprot Description
CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis (By similarity)
Protein type: Protease; Apoptosis; EC 3.4.22.36
Research Articles on Casp1
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Product Notes
The Casp1 casp1 (Catalog #AAA495223) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Caspase-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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