Rabbit anti-Human GUCY1A3 Polyclonal Antibody | anti-GUCY1A3 antibody

GUCY1A3, NT (Guanylate Cyclase Soluble Subunit alpha-3, GCS-alpha-3, Soluble Guanylate Cyclase Large Subunit, GCS-alpha-1, GUC1A3, GUCSA3, GUCY1A1) (Biotin)

Cat. Num. AAA6479355

• Gene Names: GUCY1A1; GUCA3; MYMY6; GC-SA3; GUC1A3; GUCSA3; GUCY1A3; GCS-alpha-3; GC-S-alpha-1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: GUCY1A3; Polyclonal Antibody; NT (Guanylate Cyclase Soluble Subunit alpha-3; GCS-alpha-3; Soluble Guanylate Cyclase Large Subunit; GCS-alpha-1; GUC1A3; GUCSA3; GUCY1A1) (Biotin); anti-GUCY1A3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GUCY1A3.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 690

• Applicable Applications for anti-GUCY1A3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human GUCY1A3 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GUCY1A3 (arrow) using GUCY1A3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GUCY1A3 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of anti-GUCY1A3 Pab in mouse brain tissue lysate (35ug/lane). GUCY1A3(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-GUCY1A3 antibody

Antibodies; Enzymes; Cyclase

References

1. Saino, M., et al., Oncol. Rep. 12(1):47-52 (2004). 2. Zhou, Y., et al., Gene 245(2):319-328 (2000). 3. Papapetropoulos, A., et al., J. Cell. Physiol. 167(2):213-221 (1996). 4. Giuili, G., et al., Hum. Genet. 91(3):257-260 (1993). 5. Giuili, G., et al., FEBS Lett. 304(1):83-88 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 67763816
• NCBI GeneID: 2982
• NCBI Accession #: NP_000847
• NCBI GenBank Nucleotide #: NP_000847.2
• UniProt Accession #: Q02108
• Molecular Weight: 77.452
• NCBI Official Full Name: guanylate cyclase soluble subunit alpha-1 isoform A
• NCBI Official Synonym Full Names: guanylate cyclase 1 soluble subunit alpha 1
• NCBI Official Symbol: GUCY1A1
• NCBI Official Synonym Symbols: GUCA3; MYMY6; GC-SA3; GUC1A3; GUCSA3; GUCY1A3; GCS-alpha-3; GC-S-alpha-1
• NCBI Protein Information: guanylate cyclase soluble subunit alpha-1
• UniProt Protein Name: Guanylate cyclase soluble subunit alpha-3
• Protein Family: Guanylate cyclase soluble
• UniProt Gene Name: GUCY1A3
• UniProt Synonym Gene Names: GUC1A3; GUCSA3; GUCY1A1; GCS-alpha-3
• UniProt Entry Name: GCYA3_HUMAN

NCBI Description

Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]