Western Blot (WB)
(All lanes : Anti-GTF2I Antibody (C-term) at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: A431 whole cell lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 112 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit GTF2I Polyclonal Antibody | anti-GTF2I antibody
GTF2I Antibody (C-term)
Predicted: Rat
Predicted: Rat
IF: 1:25
Western Blot (WB)
(All lanes : Anti-GTF2I Antibody (C-term) at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: A431 whole cell lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 112 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GTF2I Antibody (C-term) at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: A431 whole cell lysatesLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 112 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western blot analysis of lysates from Hela,A431 cell line (from left to right),using GTF2I Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of lysates from Hela,A431 cell line (from left to right),using GTF2I Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with GTF2I Antibody (C-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with GTF2I Antibody (C-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Background: GTF2I is a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]
Uniprot Description
GTF2I: a multifunctional phosphoprotein with roles in transcription and signal transduction. Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and Phox1 and interacts cooperatively with Phox1 to promote serum-inducible transcription of a reporter gene driven by the C- FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Its gene is known to contain at least 34 exons. Four splice variant isoforms have been described.
Protein type: Transcription, coactivator/corepressor; DNA-binding
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: nucleoplasm; cell projection; membrane; cell soma; cytoplasm; nucleus
Molecular Function: protein binding; DNA binding; mitogen-activated protein kinase binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; transition between slow and fast fiber; reduction of cytosolic calcium ion concentration; signal transduction
Disease: Williams-beuren Syndrome
Research Articles on GTF2I
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Product Notes
The GTF2I gtf2i (Catalog #AAA9231597) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GTF2I Antibody (C-term) reacts with Human Predicted: Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GTF2I can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA( EIA). WB: 1:1000 IF: 1:25. Researchers should empirically determine the suitability of the GTF2I gtf2i for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF2I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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