Western Blot (WB)
(Western Blot analysis of COS7 cells using GTBP Polyclonal Antibody)
Rabbit GTBP Polyclonal Antibody | anti-GTBP antibody
GTBP Polyclonal Antibody
Immunohistochemistry: 1:100 - 1:300
Immunofluorescence: 1:200 - 1:1000
ELISA: 1:20000
Not yet tested in other applications.
Western Blot (WB)
(Western Blot analysis of COS7 cells using GTBP Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of COS7 cells using GTBP Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of A549 cells using GTBP Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of A549 cells using GTBP Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]
Uniprot Description
MSH6: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Heterodimer consisting of MSH2-MSH6 (MutS alpha). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Belongs to the DNA mismatch repair MutS family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 2p16
Cellular Component: nucleoplasm; Golgi apparatus; nuclear chromosome; intracellular membrane-bound organelle; nuclear chromatin; cytoplasm; plasma membrane; MutSalpha complex
Molecular Function: DNA-dependent ATPase activity; protein homodimerization activity; single thymine insertion binding; oxidized purine DNA binding; ATPase activity; magnesium ion binding; ADP binding; methylated histone residue binding; mismatched DNA binding; protein binding; four-way junction DNA binding; single guanine insertion binding; guanine/thymine mispair binding; double-stranded DNA binding; MutLalpha complex binding; chromatin binding; ATP binding
Biological Process: positive regulation of helicase activity; negative regulation of DNA recombination; mismatch repair; DNA damage response, signal transduction resulting in induction of apoptosis; meiotic recombination; somatic hypermutation of immunoglobulin genes; isotype switching; somatic recombination of immunoglobulin gene segments; determination of adult life span; DNA repair; maintenance of DNA repeat elements; meiotic mismatch repair; response to UV
Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 5; Endometrial Cancer; Mismatch Repair Cancer Syndrome
Research Articles on GTBP
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Product Notes
The GTBP msh6 (Catalog #AAA9401469) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GTBP Polyclonal Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's GTBP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:300 Immunofluorescence: 1:200 - 1:1000 ELISA: 1:20000 Not yet tested in other applications. Researchers should empirically determine the suitability of the GTBP msh6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTBP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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