Western Blot (WB)
(Anti-GSTT2 rabbit polyclonal antibody at 1:500 dilutionLane A: Rat kidney tissue lysateLane B: Rat lung tissue lysateLane C: Rat pancreas tissue lysateLane D: Mouse lung tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa)
Rabbit anti-Human GSTT2B Polyclonal Antibody | anti-GSTT2B antibody
Anti-GSTT2B Antibody, Rabbit Polyclonal
IP: 1-5uL/mg of lysate
Western Blot (WB)
(Anti-GSTT2 rabbit polyclonal antibody at 1:500 dilutionLane A: Rat kidney tissue lysateLane B: Rat lung tissue lysateLane C: Rat pancreas tissue lysateLane D: Mouse lung tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa)
Western Blot (WB)
(Anti-GSTT2 rabbit polyclonal antibody at 1:500 dilutionLane A: Rat kidney tissue lysateLane B: Rat lung tissue lysateLane C: Rat pancreas tissue lysateLane D: Mouse lung tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:27 kDa)
Immunoprecipitation (IP)
(GSTT2 was immunoprecipitated using: Lane A:0.5 mg Rat lung tissue Lysate 4 uL anti-GSTT2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-GSTT2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa Observed band size :25 kDa)
Immunoprecipitation (IP)
(GSTT2 was immunoprecipitated using: Lane A:0.5 mg Rat lung tissue Lysate 4 uL anti-GSTT2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-GSTT2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa Observed band size :25 kDa)
NCBI and Uniprot Product Information
NCBI Description
Glutathione S-transferase (GSTs) theta 2 (GSTT2) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: Alpha, Mu, Pi, Theta, and Zeta. The theta class members GSTT1 and GSTT2 share 55% amino acid sequence identity and both are thought to have an important role in human carcinogenesis. The theta genes have a similar structure, being composed of five exons with identical exon/intron boundaries. [provided by RefSeq, Jul 2008]
Uniprot Description
GST2: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a sulfatase activity. Belongs to the GST superfamily. Theta family.
Protein type: EC 2.5.1.18
Chromosomal Location of Human Ortholog: 22q11.23
Cellular Component: cytoplasm
Molecular Function: glutathione transferase activity
Biological Process: metabolic process
Research Articles on GSTT2B
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Product Notes
The GSTT2B gstt2 (Catalog #AAA8107615) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GSTT2B Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GSTT2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the GSTT2B gstt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSTT2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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