Rabbit anti-Human GSTT1 Polyclonal Antibody | anti-GSTT1 antibody

GSTT1, NT (GSTT1, Glutathione S-transferase theta-1, GST class-theta-1, Glutathione transferase T1-1) (PE)

Cat. Num. AAA6304766

• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: GSTT1; Polyclonal Antibody; NT (GSTT1; Glutathione S-transferase theta-1; GST class-theta-1; Glutathione transferase T1-1) (PE); Glutathione transferase T1-1; anti-GSTT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GSTT1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-GSTT1 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 7-34 in the N-terminal region of human GSTT1, UniProt Accession #P30711.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS648983 (arrow). 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GSTT1 gene.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells (right histogram) using MBS648983 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GSTT1 antibody

Antibodies; GST

References

1. Palli, D., et al. Mutagenesis 25(6):569-575(2010). 2. Henderson, A.J., et al. Thorax 65(10):897-902(2010). 3. Filonzi, L., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):743-747(2010). 4. Smith, G., et al. Pharmacogenet. Genomics (2010). In press 5. Bid, H.K., et al. J Postgrad Med 56(3):176-181(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 167466164
• NCBI GeneID: 2952
• NCBI Accession #: NP_000844.2
• NCBI GenBank Nucleotide #: NM_000853.3
• UniProt Accession #: P30711; O00226; Q5TZY2; Q6IC69; Q969K8; Q96IY3
• Molecular Weight: 13,517 Da
• NCBI Official Full Name: glutathione S-transferase theta-1 isoform a
• NCBI Official Synonym Full Names: glutathione S-transferase theta 1
• NCBI Official Symbol: GSTT1
• NCBI Protein Information: glutathione S-transferase theta-1; GST class-theta-1; glutathione transferase T1-1
• UniProt Protein Name: Glutathione S-transferase theta-1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTT1
• UniProt Entry Name: GSTT1_HUMAN

NCBI Description

The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. GSTT1 and GSTT2 share 55% amino acid sequence identity and both may play an important role in human carcinogenesis. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries. This GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes, which are also located on chromosome 22, have been identified. [provided by RefSeq, Jun 2014]

Uniprot Description

GSTT1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide. Belongs to the GST superfamily. Theta family.

Protein type: EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Transferase; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: cytosol

Molecular Function: glutathione transferase activity; glutathione peroxidase activity

Biological Process: glutathione metabolic process; xenobiotic metabolic process