Rabbit anti-Human GSTT1 Polyclonal Antibody | anti-GSTT1 antibody

GSTT1, NT (GSTT1, Glutathione S-transferase theta-1, GST class-theta-1, Glutathione transferase T1-1)

Cat. Num. AAA648983

• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: GSTT1; Polyclonal Antibody; NT (GSTT1; Glutathione S-transferase theta-1; GST class-theta-1; Glutathione transferase T1-1); Anti -GSTT1; anti-GSTT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-GSTT1 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Flow Cytometry: 1:10-50
• Immunogen: GSTT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-34 amino acids from the N-terminal region of human GSTT1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS648983 (arrow). 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GSTT1 gene.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells (right histogram) using MBS648983 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GSTT1 antibody

Glutathione S-transferase (GST) theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.

Product Categories/Family for anti-GSTT1 antibody

Antibodies; Abs to GST

NCBI and Uniprot Product Information

• NCBI GI #: 167466164
• NCBI GeneID: 2952
• NCBI Accession #: NP_000844.2
• NCBI GenBank Nucleotide #: NM_000853.2
• UniProt Accession #: P30711; O00226; Q5TZY2; Q969K8; Q96IY3
• Molecular Weight: 27,335 Da
• NCBI Official Full Name: glutathione S-transferase theta-1
• NCBI Official Synonym Full Names: glutathione S-transferase theta 1
• NCBI Official Symbol: GSTT1
• NCBI Protein Information: glutathione S-transferase theta-1; GST class-theta-1; glutathione transferase T1-1
• UniProt Protein Name: Glutathione S-transferase theta-1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTT1
• UniProt Entry Name: GSTT1_HUMAN

NCBI Description

Glutathione S-transferase (GST) theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide. Ref.10 Ref.12

Catalytic activity: RX + glutathione = HX + R-S-glutathione. Ref.10 Ref.12

Subunit structure: Homodimer. Ref.12

Subcellular location: Cytoplasm.

Tissue specificity: Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles. Ref.9

Polymorphism: The GSTT1 gene is absent from 38% of the population. The presence or absence of the GSTT1 gene is coincident with the conjugator (GSST1+) and non-conjugator (GSTT1-) phenotypes respectively. The GSTT1+ phenotype can catalyze the glutathione conjugation of dichloromethane.

Sequence similarities: Belongs to the GST superfamily. Theta family.Contains 1 GST C-terminal domain.Contains 1 GST N-terminal domain.