Western Blot (WB)
((0.3ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Gsta5 Polyclonal Antibody | anti-Gsta5 antibody
Goat anti-Gsta5 (rat) Antibody
Gene Names
Gsta3; Yc2; Gsta5
Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Gsta5; Polyclonal Antibody; Goat anti-Gsta5 (rat) Antibody; glutathione S-transferase Yc2 subunit; MGC112704; Yc2; GST A5-5; GST Yc2; glutathione S-transferase Yc-2; glutathione S-transferase alpha-5; Gsta5 antibody; glutathione S-transferase Yc2 subunit antibody; MGC112704 antibody; Yc2 antibody; GST A5-5 antibody; GST Yc2 antibody; glutathione S-transferase Yc-2 antibody; glutathione S-transferase alpha-5 antibody; Gsta5 (rat); anti-Gsta5 antibody
Host
Goat
Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EMDPGIVDNFPLLK
Sequence Length
221
Applicable Applications for anti-Gsta5 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 25kDa band observed in Mouse and Rat lysates (calculated MW of 25.3kDa according to NP_001009920.1. Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 25kDa band observed in Mouse and Rat lysates (calculated MW of 25.3kDa according to NP_001009920.1. Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-EMDPGIVDNFPLLK, from the internal region of the protein sequence according to NP_001009920.1.
Epitope
Internal region
Note
Reported variants represent identical protein: NP_001009920.1, NP_001153211.1. Please note that this antibody is expected to cross-react to the homologous Mouse protein Gsta3
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,347 Da
NCBI Official Full Name
glutathione S-transferase alpha-5 isoform 1
NCBI Official Synonym Full Names
glutathione S-transferase alpha 3
NCBI Official Symbol
Gsta3
NCBI Official Synonym Symbols
Yc2; Gsta5
NCBI Protein Information
glutathione S-transferase alpha-5
UniProt Protein Name
Glutathione S-transferase alpha-5
Protein Family
UniProt Gene Name
Gsta5
UniProt Synonym Gene Names
Gstyc2; GST Yc2
UniProt Entry Name
GSTA5_RAT
Uniprot Description
GSTA3: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes isomerization reactions that contribute to the biosynthesis of steroid hormones. Efficiently catalyze obligatory double-bond isomerizations of delta(5)-androstene-3,17-dione and delta(5)- pregnene-3,20-dione, precursors to testosterone and progesterone, respectively. Belongs to the GST superfamily. Alpha family.
Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Xenobiotic Metabolism - metabolism by cytochrome P450; Transferase
Molecular Function: glutathione transferase activity
Biological Process: aflatoxin catabolic process
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Product Notes
The Gsta5 gsta5 (Catalog #AAA422587) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Gsta5 (rat) Antibody reacts with Tested: Mouse, Rat; Expected from sequence similarity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Gsta5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 25kDa band observed in Mouse and Rat lysates (calculated MW of 25.3kDa according to NP_001009920.1. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the Gsta5 gsta5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EMDPGIVDNF PLLK. It is sometimes possible for the material contained within the vial of "Gsta5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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