Mouse anti-Human RTKN2 Monoclonal Antibody | anti-RTKN2 antibody

Mouse Monoclonal [clone 2C2] (IgG2a,k) to Human RTKN2

Cat. Num. AAA246539

• Gene Names: RTKN2; PLEKHK1; bA531F24.1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: RTKN2; Monoclonal Antibody; Mouse Monoclonal [clone 2C2] (IgG2a; k) to Human RTKN2; Anti-RTKN2 Antibody (clone 2C2) IHC-plus; BA531F24.1; Em:AC024597.2; Rhotekin-2; PLEKHK1; Rhotekin 2; Human RTKN2; anti-RTKN2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2C2
• Specificity: Human RTKN2
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 163

• Applicable Applications for anti-RTKN2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (1:50), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot with the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: RTKN2 (AAH25765, 1 a.a. ~ 164 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: RTKN2 antibody was raised against rTKN2 (AAH25765, 1 a.a. ~ 164 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-RTKN2 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

ELISA (EIA)

(Detection limit for recombinant GST tagged RTKN2 is 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 545478494
• NCBI GeneID: 219790
• NCBI Accession #: NP_001269870.1
• NCBI GenBank Nucleotide #: NM_001282941.1
• UniProt Accession #: Q8IZC4; Q3ZCR1; Q68DZ6; Q8N8K1; Q8TAV2
• Molecular Weight: 18,655 Da
• NCBI Official Full Name: rhotekin-2 isoform 2
• NCBI Official Synonym Full Names: rhotekin 2
• NCBI Official Symbol: RTKN2
• NCBI Official Synonym Symbols: PLEKHK1; bA531F24.1
• NCBI Protein Information: rhotekin-2; PH domain-containing family K member 1; pleckstrin homology domain-containing family K member 1; pleckstrin homology domain containing, family K member 1
• UniProt Protein Name: Rhotekin-2
• Protein Family: Rhotekin
• UniProt Gene Name: RTKN2
• UniProt Synonym Gene Names: PLEKHK1; PH domain-containing family K member 1
• UniProt Entry Name: RTKN2_HUMAN

Uniprot Description

RTKN2: a Rho-GTPase effector protein with a PH domain. Expressed in CD4+ T-cells and bone marrow-derived B-cells. Gene expression was increased in quiescent T-cells and negligible in activated proliferating cells. Expression was absent in myeloid leukaemias, low in most B-cell malignancies and CD8+ T-cell malignancies, but very high in double positive T-lymphoblastic lymphoma. Three alternatively spliced variants have been reported in human samples.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 10q21.2

Cellular Component: plasma membrane

Biological Process: positive regulation of cell proliferation; hemopoiesis; signal transduction