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Western Blot (WB) (Western Blot: Sample: Recombinant GROg, Human.)

Rabbit anti-Human Growth Regulated Oncogene Gamma (GROg) Polyclonal Antibody | anti-GROg antibody

Polyclonal Antibody to Growth Regulated Oncogene Gamma (GROg)

Gene Names
CXCL3; GRO3; GROg; MIP2B; SCYB3; MIP-2b; CINC-2b
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Growth Regulated Oncogene Gamma (GROg); Polyclonal Antibody; Polyclonal Antibody to Growth Regulated Oncogene Gamma (GROg); anti-GROg antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GROg. It has been selected for its ability to recognize GROg in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGS- ASVVTE LRCQCLQTLQ GIHLKNIQSV NVRSPGPHCA QTEVIATLKN GKKACLNPAS PMVQKIIEKI LNKGSTN
Applicable Applications for anti-GROg antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GROg (Ala35~Asn107) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2057007
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant GROg, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant GROg, Human.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:HumanTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:HumanTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11,342 Da
NCBI Official Full Name
C-X-C motif chemokine 3
NCBI Official Synonym Full Names
C-X-C motif chemokine ligand 3
NCBI Official Symbol
CXCL3
NCBI Official Synonym Symbols
GRO3; GROg; MIP2B; SCYB3; MIP-2b; CINC-2b
NCBI Protein Information
C-X-C motif chemokine 3
UniProt Protein Name
C-X-C motif chemokine 3
UniProt Gene Name
CXCL3
UniProt Synonym Gene Names
GRO3; GROG; SCYB3; GRO-gamma; MIP2-beta

NCBI Description

This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL3: Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular region; extracellular space

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor signaling pathway; immune response; inflammatory response; neutrophil chemotaxis; regulation of cell proliferation; response to lipopolysaccharide

Research Articles on GROg

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Product Notes

The GROg cxcl3 (Catalog #AAA2003341) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Growth Regulated Oncogene Gamma (GROg) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Growth Regulated Oncogene Gamma (GROg) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GROg cxcl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG S- ASVVTE LRCQCLQTLQ GIHLKNIQSV NVRSPGPHCA QTEVIATLKN GKKACLNPAS PMVQKIIEKI LNKGSTN. It is sometimes possible for the material contained within the vial of "Growth Regulated Oncogene Gamma (GROg), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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