Testing Data
(The first lane is the marker which is difficult to see. 2nd lane starts the Antigen: A 1:2 dilution was used starting with 250ng/lane. The antibody was used at a concentration of 0.2pg/ml.)
Rabbit anti-Rat GRO beta Polyclonal Antibody
GRO beta (MIP-2, Macrophage Inflammatory Protein 2) (AP)
Reactivity
Rat
Applications
ELISA, Neutralization Assay, Western Blot
Purity
Purified by immobilized antigen affinity chromatography.
Synonyms
GRO beta; Polyclonal Antibody; GRO beta (MIP-2; Macrophage Inflammatory Protein 2) (AP); anti-GRO beta antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes GRO/MIP-2
Purity/Purification
Purified by immobilized antigen affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-GRO beta antibody
ELISA (EIA), Neutralization, Western Blot (WB)
Application Notes
ELISA: Detection by direct ELISA (using 100ul/well antibody solution), requires a concentration of at least 0.5ug/ml of antibody. This antigen-affinity purified antibody, in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity (100ng/ml), a concentration of 3.0-5.0ug/ml is required.
WB: Use at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Applications are based on unconjugated antibody.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity (100ng/ml), a concentration of 3.0-5.0ug/ml is required.
WB: Use at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Applications are based on unconjugated antibody.
Immunogen
Highly purified (>98%) recombinant antigen.
Conjugate
AP
Note
Preservative Free
Special Handling
Hygroscopic. Keep tightly closed and desiccated.
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Testing Data
(The first lane is the marker which is difficult to see. 2nd lane starts the Antigen: A 1:2 dilution was used starting with 250ng/lane. The antibody was used at a concentration of 0.2pg/ml.)
Testing Data
(The first lane is the marker which is difficult to see. 2nd lane starts the Antigen: A 1:2 dilution was used starting with 250ng/lane. The antibody was used at a concentration of 0.2pg/ml.)
Product Categories/Family for anti-GRO beta antibody
Similar Products
Product Notes
The GRO beta (Catalog #AAA6479133) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GRO beta (MIP-2, Macrophage Inflammatory Protein 2) (AP) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GRO beta can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Neutralization, Western Blot (WB). ELISA: Detection by direct ELISA (using 100ul/well antibody solution), requires a concentration of at least 0.5ug/ml of antibody. This antigen-affinity purified antibody, in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity (100ng/ml), a concentration of 3.0-5.0ug/ml is required. WB: Use at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GRO beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRO beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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