Immunohistochemistry (IHC)
(BRUNOL6 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BRUNOL6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human, Mouse BRUNOL6 Polyclonal Antibody | anti-BRUNOL6 antibody
BRUNOL6, NT (CELF6, BRUNOL6, CUGBP Elav-like family member 6, Bruno-like protein 6, CUG-BP-and ETR-3-like factor 6, RNA-binding protein BRUNOL-6) (Azide free) (HRP)
Gene Names
CELF6; BRUNOL6
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
BRUNOL6; Polyclonal Antibody; NT (CELF6; CUGBP Elav-like family member 6; Bruno-like protein 6; CUG-BP-and ETR-3-like factor 6; RNA-binding protein BRUNOL-6) (Azide free) (HRP); CELF6; RNA-binding protein BRUNOL-6; anti-BRUNOL6 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-BRUNOL6 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
BRUNOL6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-82 amino acids from the N-terminal region of human BRUNOL6.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(BRUNOL6 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BRUNOL6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(BRUNOL6 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BRUNOL6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Western Blot (WB)
(BRUNOL6 Antibody (N-term) western blot analysis in mouse kidney,liver tissue lysates (35ug/lane).This demonstrates the BRUNOL6 antibody detected the BRUNOL6 protein (arrow).)
Western Blot (WB)
(BRUNOL6 Antibody (N-term) western blot analysis in mouse kidney,liver tissue lysates (35ug/lane).This demonstrates the BRUNOL6 antibody detected the BRUNOL6 protein (arrow).)
Related Product Information for anti-BRUNOL6 antibody
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
Product Categories/Family for anti-BRUNOL6 antibody
References
Ladd, A.N., et al. J. Biol. Chem. 279(17):17756-17764(2004). Wistow, G., et al. Mol. Vis. 8, 205-220 (2002): Good, P.J., et al. J. Biol. Chem. 275(37):28583-28592(2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,477 Da
NCBI Official Full Name
CUGBP Elav-like family member 6 isoform 2
NCBI Official Synonym Full Names
CUGBP, Elav-like family member 6
NCBI Official Symbol
CELF6
NCBI Official Synonym Symbols
BRUNOL6
NCBI Protein Information
CUGBP Elav-like family member 6; CELF-6; bruno-like protein 6; RNA-binding protein BRUNOL-6; CUG-BP and ETR-3 like factor 6; CUG-BP- and ETR-3-like factor 6; bruno-like 6, RNA binding protein; Bruno -like 6, RNA binding protein
UniProt Protein Name
CUGBP Elav-like family member 6
UniProt Gene Name
CELF6
UniProt Synonym Gene Names
BRUNOL6; CELF-6
UniProt Entry Name
CELF6_HUMAN
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Product Notes
The BRUNOL6 celf6 (Catalog #AAA6279026) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRUNOL6, NT (CELF6, BRUNOL6, CUGBP Elav-like family member 6, Bruno-like protein 6, CUG-BP-and ETR-3-like factor 6, RNA-binding protein BRUNOL-6) (Azide free) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BRUNOL6 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BRUNOL6 celf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRUNOL6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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