Western Blot (WB)
(Figure 1. Western blot analysis of GRO alpha using anti-GRO alpha antibody (MBS1750429).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant rat CXCL1 protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRO alpha antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GRO alpha at approximately 11KD. The expected band size for GRO alpha is at 11KD. )
Rabbit GRO alpha Polyclonal Antibody | anti-Cxcl1 antibody
Anti-GRO alpha Picoband antibody
Gene Names
Cxcl1; Gro1; CINC-1
Reactivity
Mouse, RatNo cross reactivity with other proteins.
Applications
ELISA, Western Blot
Synonyms
GRO alpha; Polyclonal Antibody; Anti-GRO alpha Picoband antibody; Growth-regulated alpha protein; C-X-C motif chemokine 1; Cytokine-induced neutrophil chemoattractant 1; CINC-1; Platelet-derived growth factor-inducible protein KC; Cxcl1; Cinc1; Gro; Scyb1; anti-Cxcl1 antibody
Host
Rabbit
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Clonality
Polyclonal
Form/Format
Lyophilized
Sequence Length
96
Applicable Applications for anti-Cxcl1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 0.1-0.5mug/ml
ELISA(Cap): 1-5mug/ml
ELISA(Cap): 1-5mug/ml
Immunogen
E Coli-derived rat GRO alpha recombinant protein (Position: A25-K96).
Subcellular Localization
Secreted.
Tissue Specificity
At least expressed in the lung and trachea.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of GRO alpha using anti-GRO alpha antibody (MBS1750429).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant rat CXCL1 protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRO alpha antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GRO alpha at approximately 11KD. The expected band size for GRO alpha is at 11KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of GRO alpha using anti-GRO alpha antibody (MBS1750429).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant rat CXCL1 protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRO alpha antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GRO alpha at approximately 11KD. The expected band size for GRO alpha is at 11KD. )
Related Product Information for anti-Cxcl1 antibody
Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,249 Da
NCBI Official Full Name
growth-regulated alpha protein
NCBI Official Synonym Full Names
C-X-C motif chemokine ligand 1
NCBI Official Symbol
Cxcl1
NCBI Official Synonym Symbols
Gro1; CINC-1
NCBI Protein Information
growth-regulated alpha protein
UniProt Protein Name
Growth-regulated alpha protein
Protein Family
UniProt Gene Name
Cxcl1
UniProt Synonym Gene Names
Cinc1; Gro; Scyb1; CINC-1
NCBI Description
acts as a neutrophil chemoattractant; may play a role in acute phase inflammatory response [RGD, Feb 2006]
Uniprot Description
Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation.
Research Articles on Cxcl1
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Product Notes
The Cxcl1 cxcl1 (Catalog #AAA1750429) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GRO alpha Picoband antibody reacts with Mouse, Rat No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's GRO alpha can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 0.1-0.5mug/ml ELISA(Cap): 1-5mug/ml. Researchers should empirically determine the suitability of the Cxcl1 cxcl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRO alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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