Rabbit anti-Human GRIN3B Polyclonal Antibody | anti-GRIN3B antibody

GRIN3B, CT (GRIN3B, Glutamate [NMDA] receptor subunit 3B, N-methyl-D-aspartate receptor subtype 3B) (Azide free) (HRP)

Cat. Num. AAA6304452

• Gene Names: GRIN3B; NR3B; GluN3B
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GRIN3B; Polyclonal Antibody; CT (GRIN3B; Glutamate [NMDA] receptor subunit 3B; N-methyl-D-aspartate receptor subtype 3B) (Azide free) (HRP); N-methyl-D-aspartate receptor subtype 3B; anti-GRIN3B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GRIN3B antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GRIN3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 903-933 amino acids from the C-terminal region of human GRIN3B.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GRIN3B Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GRIN3B antibody detected the GRIN3B protein (arrow).)

Immunohistochemistry (IHC)

(GRIN3B Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GRIN3B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-GRIN3B antibody

GRIN3B is a NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. It is mediated by glycine.

Product Categories/Family for anti-GRIN3B antibody

Antibodies; Ion Channel

References

Sedaghati, M., et al. Eur. J. Pharmacol. 633 (1-3), 50-54 (2010): Saus, E., et al. J Psychiatr Res (2010). In press: Need, A.C., et al. Eur. J. Hum. Genet. 17(7):946-957(2009). Liu, H.P., et al. Dement Geriatr Cogn Disord 28(6):521-527(2009). Niemann, S., et al. Neurology 70(9):666-676(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 62988346
• NCBI GeneID: 116444
• NCBI Accession #: NP_619635.1
• NCBI GenBank Nucleotide #: NM_138690.2
• UniProt Accession #: O60391; Q5EAK7; Q7RTW9
• Molecular Weight: 112,992 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 3B
• NCBI Official Synonym Full Names: glutamate ionotropic receptor NMDA type subunit 3B
• NCBI Official Symbol: GRIN3B
• NCBI Official Synonym Symbols: NR3B; GluN3B
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 3B
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 3B
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRIN3B
• UniProt Synonym Gene Names: GluN3B; NMDAR3B; NR3B
• UniProt Entry Name: NMD3B_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of an N-methyl-D-aspartate (NMDA) receptor. The encoded protein is found primarily in motor neurons, where it forms a heterotetramer with GRIN1 to create an excitatory glycine receptor. Variations in this gene have been proposed to be linked to schizophrenia. [provided by RefSeq, Nov 2015]

Uniprot Description

NMDAR3B: NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR3B/GRIN3B subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: postsynaptic membrane; cell soma; dendrite; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: neurotransmitter binding; extracellular-glutamate-gated ion channel activity; calcium channel activity; N-methyl-D-aspartate selective glutamate receptor activity; glycine binding; cation channel activity; neurotransmitter receptor activity

Biological Process: synaptic transmission, glutamatergic; ionotropic glutamate receptor signaling pathway; regulation of calcium ion transport; protein insertion into membrane