Rabbit anti-Mouse GRIN2A Polyclonal Antibody | anti-GRIN2A antibody

GRIN2A, ID (GRIN2A, NMDAR2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A) (FITC)

Cat. Num. AAA6304429

• Gene Names: GRIN2A; LKS; EPND; FESD; NR2A; GluN2A; NMDAR2A
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GRIN2A; Polyclonal Antibody; ID (GRIN2A; NMDAR2A; Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A) (FITC); N-methyl D-aspartate receptor subtype 2A; anti-GRIN2A antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GRIN2A antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GRIN2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1064-1092 amino acids from the Central region of human GRIN2A.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GRIN2A Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the GRIN2A antibody detected the GRIN2A protein (arrow).)

Immunohistochemistry (IHC)

(GRIN2A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GRIN2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-GRIN2A antibody

Antibodies; Ion Channel

References

Endele, S., et al. Nat. Genet. 42(11):1021-1026(2010). Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Saus, E., et al. J Psychiatr Res 44(14):971-978(2010). Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010): King, J.E., et al. Am. J. Pathol. 176(6):2819-2830(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 197313638
• NCBI GeneID: 2903
• NCBI Accession #: NP_001127880.1; NP_001127879.1
• NCBI GenBank Nucleotide #: NM_001134408.2
• UniProt Accession #: Q12879; O00669; Q17RZ6
• Molecular Weight: 144,431 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2A isoform 2
• NCBI Official Synonym Full Names: glutamate ionotropic receptor NMDA type subunit 2A
• NCBI Official Symbol: GRIN2A
• NCBI Official Synonym Symbols: LKS; EPND; FESD; NR2A; GluN2A; NMDAR2A
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2A
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2A
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRIN2A
• UniProt Synonym Gene Names: NMDAR2A; GluN2A; NMDAR2A; NR2A; hNR2A
• UniProt Entry Name: NMDE1_HUMAN

NCBI Description

This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission.

Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p13.2

Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; cell surface; endoplasmic reticulum; integral to plasma membrane; dendrite; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; zinc ion binding; N-methyl-D-aspartate selective glutamate receptor activity; calcium channel activity

Biological Process: startle response; positive regulation of apoptosis; regulation of synaptic plasticity; sensory perception of pain; dopamine metabolic process; synaptic transmission; protein localization; learning and/or memory; transport; response to wounding; visual learning; serotonin metabolic process; response to drug; synaptic transmission, glutamatergic; glutamate signaling pathway; response to amphetamine; sleep; memory; response to ethanol; neurogenesis; directional locomotion; ionotropic glutamate receptor signaling pathway; regulation of sensory perception of pain; negative regulation of protein catabolic process; regulation of excitatory postsynaptic membrane potential

Disease: Epilepsy, Focal, With Speech Disorder And With Or Without Mental Retardation