Human GRIN2A ELISA Kit | GRIN2A elisa kit

Human GRIN2A (Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A) ELISA Kit

Cat. Num. AAA2516108

• Gene Names: GRIN2A; LKS; EPND; FESD; NR2A; GluN2A; NMDAR2A
• Reactivity: Human
• Synonyms: GRIN2A; Human GRIN2A (Glutamate Receptor; Ionotropic; N-Methyl-D-Aspartate 2A) ELISA Kit; GRIN2A elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human GRIN2A. No significant cross-reactivity or interference between Human GRIN2A and analogues was observed.
• Sequence Length: 1281

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human GRIN2A concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRIN2A elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GRIN2A. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GRIN2A and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GRIN2A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GRIN2A. You can calculate the concentration of GRIN2A in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 197313638
• NCBI GeneID: 2903
• NCBI Accession #: NP_001127880.1
• NCBI GenBank Nucleotide #: NM_001134408.2
• Molecular Weight: 144,431 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2A isoform 2
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• NCBI Official Symbol: GRIN2A
• NCBI Official Synonym Symbols: LKS; EPND; FESD; NR2A; GluN2A; NMDAR2A
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor channel, subunit epsilon-1; N-methyl-D-aspartate receptor subunit 2A
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2A
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRIN2A
• UniProt Synonym Gene Names: NMDAR2A; GluN2A; NMDAR2A; NR2A; hNR2A
• UniProt Entry Name: NMDE1_HUMAN

NCBI Description

This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission.

Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p13.2

Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; cell surface; endoplasmic reticulum; integral to plasma membrane; dendrite; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; zinc ion binding; N-methyl-D-aspartate selective glutamate receptor activity; calcium channel activity

Biological Process: response to drug; synaptic transmission, glutamatergic; startle response; glutamate signaling pathway; positive regulation of apoptosis; response to amphetamine; regulation of synaptic plasticity; sleep; sensory perception of pain; dopamine metabolic process; memory; synaptic transmission; protein localization; learning and/or memory; response to ethanol; neurogenesis; directional locomotion; transport; ionotropic glutamate receptor signaling pathway; response to wounding; visual learning; regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; negative regulation of protein catabolic process; serotonin metabolic process

Disease: Epilepsy, Focal, With Speech Disorder And With Or Without Mental Retardation