Western Blot (WB)
(Western blot analysis of extracts of SH-SY5Y cells, using GRHL2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Rabbit anti-Human GRHL2 Polyclonal Antibody | anti-GRHL2 antibody
GRHL2 Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of SH-SY5Y cells, using GRHL2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Western Blot (WB)
(Western blot analysis of extracts of SH-SY5Y cells, using GRHL2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
NCBI and Uniprot Product Information
Molecular Weight: 625
NCBI Description
The protein encoded by this gene is a transcription factor that can act as a homodimer or as a heterodimer with either GRHL1 or GRHL3. Defects in this gene are a cause of non-syndromic sensorineural deafness autosomal dominant type 28 (DFNA28).[provided by RefSeq, Mar 2009]
Uniprot Description
GRHL2: May function as a transcription factor. Defects in GRHL2 are the cause of deafness autosomal dominant type 28 (DFNA28). DFNA28 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNA28 is characterized by mild to moderate hearing loss across most frequencies that progressed to severe loss in the higher frequencies by the fifth decade. Age at onset varied, with the earliest case documented at 7 years of age. Belongs to the grh/CP2 family. Grainyhead subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 8q22.3
Cellular Component: intercellular junction; nucleoplasm; nucleus; plasma membrane
Molecular Function: chromatin DNA binding; protein binding; sequence-specific DNA binding; transcription factor activity
Biological Process: brain development; camera-type eye development; cell adhesion; cell proliferation; embryonic cranial skeleton morphogenesis; embryonic digit morphogenesis; epidermis development; multicellular organism growth; negative regulation of keratinocyte differentiation; neural tube closure; neural tube development; positive regulation of telomerase activity; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
Disease: Deafness, Autosomal Dominant 28; Ectodermal Dysplasia/short Stature Syndrome
Research Articles on GRHL2
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Product Notes
The GRHL2 grhl2 (Catalog #AAA9129242) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GRHL2 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GRHL2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the GRHL2 grhl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRHL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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