Rabbit Green Fluorescent Protein Polyclonal Antibody | anti-GFP antibody
Rabbit anti-Green Fluorescent Protein (GFP native)
Reactivity
Aequoria Victoria (native) GFP, Recombinant (E Coli) and native GFP-fusion proteins, and all variants of GFP tested.
Applications
Western Blot, ELISA
Synonyms
Green Fluorescent Protein; Polyclonal Antibody; Rabbit anti-Green Fluorescent Protein (GFP native); anti-GFP antibody
Host
Rabbit
Reactivity
Aequoria Victoria (native) GFP, Recombinant (E Coli) and native GFP-fusion proteins, and all variants of GFP tested.
Clonality
Polyclonal
Specificity
The GFP antiserum is directed against native green fluorescent protein from A.victoria jellyfish. The antibody recognises not only the native GFP, but also recombinant protein (E Coli), native GFP-fusion proteins and all variants of GFP tested. Cross reactivity with E Coli. proteins is minimal.
Form/Format
Each vial contains 100ul 1 mg/ml polyclonal antiserum in 10 mM TRIS buffer pH 7.2, containing 0.02% sodium azide. The antibodies were purified by ammonium sulfate precipitation.
Sequence Length
238
Applicable Applications for anti-GFP antibody
Western Blot (WB), ELISA (EIA)
Application Notes
The antibody is suitable for the detection of GFP by western blotting and ELISA.Recommended dilution for ELISA: 1:2,000 to 1:10,000. For immunoblotting a dilution of 1:1,000 to 1:5,000 is recommended.
Source Note
The anti-GFP antibody is a rabbit polyclonal antiserum obtained from rabbits immunised with highly purified native GFP derived from Aequorea victoria jellyfish.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Related Product Information for anti-GFP antibody
Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research and embryology, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols.
References
1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
NCBI and Uniprot Product Information
Uniprot Description
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.
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Product Notes
The GFP gfp (Catalog #AAA573776) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Green Fluorescent Protein (GFP native) reacts with Aequoria Victoria (native) GFP, Recombinant (E Coli) and native GFP-fusion proteins, and all variants of GFP tested. and may cross-react with other species as described in the data sheet. AAA Biotech's Green Fluorescent Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). The antibody is suitable for the detection of GFP by western blotting and ELISA.Recommended dilution for ELISA: 1:2,000 to 1:10,000. For immunoblotting a dilution of 1:1,000 to 1:5,000 is recommended. Researchers should empirically determine the suitability of the GFP gfp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Green Fluorescent Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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