Chicken Green Fluorescent Protein Polyclonal Antibody | anti-GFP antibody

Green Fluorescent Protein (GFP) (Biotin)

Cat. Num. AAA6478981

• Applications: Immunocytochemistry, Western Blot, Immunoprecipitation
• Purity: Purified
• Synonyms: Green Fluorescent Protein; Polyclonal Antibody; Green Fluorescent Protein (GFP) (Biotin); anti-GFP antibody

• Host: Chicken
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes Green Fluorescent Protein (GFP). Has been used to detect GFP in primary neurons and zebrafish fibroblasts. Reactive with GFP from both native and recombinant sources. Species Crossreactivity: vertebrates
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GFP antibody: Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: ICC: Cells were fixed using 4% formaldehyde in PBS, pH 7.4 for 20 minutes at RT. The cells are permeabilized with 0.2% Triton X-100 and 2% serum (corresponding to the animal in which the secondary antibody was raised) in PBS for 20 minutes at RT. Cell cultures are then incubated with primary antisera in PBS containing 2% serum (as above), at 4 degree C, overnight. Has also been used successfully with the avidin/biotin/peroxidase detection system. Cultures were washed four times (10 minutes each) between steps, and reaction product is developed with a 0.05% diaminobenzidine/ 0.02% hydrogen peroxide solution on ice for 8-10 minutes.; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to Green Fluorescent Protein (GFP) containing a 6-His tag.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemistry of cells transfected with GFP expression vectors. Cells were transfected with DNA containing either a zebrafish EF1 promoter directing transcription of a human tau-GFP fusion protein or a CMV promoter directing expression of eGFP. Cells were fixed, permeabilized, incubated with G8965-09 (1:2500), followed by incubation with HRP-conjugated anti-chicken secondary antibody. Color was developed by incubating with DAB/peroxide.)

Product Categories/Family for anti-GFP antibody

Antibodies; Epitope Tag; GFP

References

1. Hallam, S.J., et al., Nature Neuroscience 5: 1137-1146 (2002). 2. Sippy, T., et al., Nature Neuroscience 10: 1031-1038 (2003). 3. Jakobsson, J., et al., European Journal of Neuroscience 19: 761-765 (2004). 4. Zhao, S., et al., European Journal of Neuroscience 19: 1133-1140 (2004). 5. Yawata, S., et al., Journal of Neuroscience 26: 3626-3633 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 1169893
• UniProt Accession #: P42212; Q17104; Q27903; Q93125
• NCBI Official Full Name: Green fluorescent protein
• UniProt Protein Name: Green fluorescent protein
• Protein Family: Green fluorescent protein
• UniProt Gene Name: GFP
• UniProt Entry Name: GFP_AEQVI

Uniprot Description

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.