Rabbit Green Fluorescent Polyclonal Antibody

Rabbit anti-native Green Fluorescent Protein (Immunoaffinity purified)

Cat. Num. AAA397405

• Reactivity: Aequoria Victoria (Native) GFP, Recombinant (E.Coli) and Native GFP-Fusion Proteins, and All Variants of GFP tested.
• Applications: Western Blot, ELISA, Immunocytochemistry
• Purity: Immunoaffinity purified
• Synonyms: Green Fluorescent; Polyclonal Antibody; Rabbit anti-native Green Fluorescent Protein (Immunoaffinity purified); anti-Green Fluorescent antibody

• Host: Rabbit
• Reactivity: Aequoria Victoria (Native) GFP, Recombinant (E.Coli) and Native GFP-Fusion Proteins, and All Variants of GFP tested.
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: The GFP antiserum is directed against native green fluorescent protein from A.victoria jellyfish. The antibody recognises not only the native GFP, but also recombinant protein (E.Coli), native GFP-fusion proteins and all variants of GFP tested. Cross reactivity with E.coli. proteins is minimal.
• Purity/Purification: Immunoaffinity purified
• Form/Format: Antiserum in 10 mM TRIS buffer pH 8.0, containing 0.02% sodium azide.

• Applicable Applications for anti-Green Fluorescent antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:5,000 to 1:25,000; Immunoblotting: 1:2,000 to 1:10,000; Immunocytochemistry the antiserum can be diluted 1:100 to 1:250.
• Source Note: The anti-GFP antibody is a rabbit polyclonal antiserum obtained from rabbits immunised with highly purified native GFP derived from Aequorea victoria jellyfish
• Preparation and Storage: The antibody is shipped at ambient temperature and may be stored at +4 degree C. For prolonged storage prepare appropriate aliquots and store at or below -20 degree C.; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2).; Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Related Product Information for anti-Green Fluorescent antibody

Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research, embryology, and plant sciences, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols. The antibody is also suitable for immunocytochemical detection of proteins labeled with the different variants of GFP.

References

1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805.