Rabbit anti-Mouse, Rat Grb14 Polyclonal Antibody | anti-GRB14 antibody

Grb14 (Growth Receptor-bound Protein 14)

Cat. Num. AAA613093

• Gene Names: GRB14; GRB14
• Reactivity: Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Serum; Serum
• Synonyms: Grb14; Polyclonal Antibody; Grb14 (Growth Receptor-bound Protein 14); Anti -Grb14 (Growth Receptor-bound Protein 14); anti-GRB14 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Growth Receptor-bound Protein 14. No significant sequence homology exist with other Grb7 family members.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in PBS, 0.05% sodium azide.

• Applicable Applications for anti-GRB14 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:10,000-50,000. Control peptide ; an be used to coat ELISA plates at 1ug/ml.; Western Blot: 1:1000-1:5000
• Immunogen: A 16aa synthetic peptide ~ within the N-temrinus of rat Grb14 (KLH). Species Sequence Homology: mouse-100%, human-87%. Cellular Localization: Cytoplasm/Golgi apparatus membrane (by similarity), Endosome membrane - Peripheral membrane protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-GRB14 antibody

Tankyrases (TRF1 interacting ankyrin-related ADP-ribose polymerase; human 1327 aa, renamed as TNKS-1/TANK1, chromosome 8, and its homolog TANK2), a modular protein with homology to ankyrin and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere. In addition to regulating the telomere and nuclear localization, TANKs also resides at the other subcellular compartments such as nuclear envelope, specifically on cytoplasmic fibers of nuclear pore complexes. TANK2 has also been shown to reside in the low-density microsomes and associate with the adapter protein, Grb14. The N-terminal 110aa of Grb14 and 10-19 ANK-repeats of TANK2 mediate this interaction. Grb14 (mouse/rat 538aa, human 540aa, chromosome 2) is a member of Grb7 (Growth receptor bound proteins) family that includes Grb7, and Grb10. Grb family is characterized by the presence of a conserved, short non-catalytic SH2 domain (Src homology region 2) that binds to peptide sequences containing phopshotyrosine. Many intracellular targets of receptor tyrosine kinases contain 1 or more SH2 domains. Grb14 is widely distributed in many tissues with an abundance in testis, ovary, heart, liver, skeletal muscle, kidney and pancreas. TANKs may play a role vesicle trafficking and in cytoplasmic signal transduction pathways via the Grg14.

Product Categories/Family for anti-GRB14 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 71979663
• NCBI GeneID: 2888
• NCBI Accession #: NP_004481.2
• NCBI GenBank Nucleotide #: NM_004490.2
• UniProt Accession #: Q14449; Q7Z6I1; Q53QM9; Q53QQ0
• Molecular Weight: 60,988 Da
• NCBI Official Full Name: growth factor receptor-bound protein 14
• NCBI Official Synonym Full Names: growth factor receptor-bound protein 14
• NCBI Official Symbol: GRB14
• NCBI Official Synonym Symbols: GRB14
• NCBI Protein Information: growth factor receptor-bound protein 14; OTTHUMP00000163009; OTTHUMP00000204702; GRB14 adapter protein
• UniProt Protein Name: Growth factor receptor-bound protein 14
• Protein Family: Growth factor receptor-bound protein
• UniProt Gene Name: GRB14
• UniProt Entry Name: GRB14_HUMAN

NCBI Description

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Transcript variants have been reported for this gene, but their full-length natures have not been determined to date. [provided by RefSeq]

Uniprot Description

GRB14: Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal. Belongs to the GRB7/10/14 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2q22-q24

Cellular Component: Golgi membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; endosome membrane; cytosol

Molecular Function: SH3/SH2 adaptor activity

Biological Process: positive regulation of signal transduction; blood coagulation; signal transduction; leukocyte migration