Rabbit GRASP55 Polyclonal Antibody | anti-GRASP55 antibody

GRASP55 (L215) Polyclonal Antibody

Cat. Num. AAA3003615

• Gene Names: GORASP2; p59; GRS2; GOLPH6; GRASP55
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GRASP55; Polyclonal Antibody; GRASP55 (L215) Polyclonal Antibody; Golgi reassembly-stacking protein 2; GRS2; Golgi phosphoprotein 6; GOLPH6; GORASP2; anti-GRASP55 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GRASP55 (L215) polyclonal antibody detects endogenous levels of GRASP55 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 384

• Applicable Applications for anti-GRASP55 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 180-234 of Human GRASP55.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GRASP55 (L215) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Mouse heart tissue lysateLane3:PC12 whole cell lysate)

Western Blot (WB)

(Western Blot (WB) analysis of GRASP55 (L215) pAb at 1:500 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug))

Related Product Information for anti-GRASP55 antibody

The Golgi apparatus is a highly complex organelle comprised of a stack of cisternal membranes on the secretory pathway from the ER to the cell surface. The structure is maintained by an exoskeleton or Golgi matrix constructed from a family of coiled-coil protein, the golgins and other peripheral membrane components such as GRASP55 and GRASP65. GRASP55 (Golgi reassembly stacking protien or p59) is a component of the Golgi stacking machinery. GRASP55 is highly homologous to GRASP65 and contains two PDZ domains. GRASP55 is myristoylated and palmitoylated. Unlike GRASP65, GRASP55 does not have detectable binding with the vesicle docking protein GM130 and is located on the medial-Golgi rather than cis-Golgi. Both GRASP55 and GRASP65 function in the stacking of Golgi cisternae. The novel coiled-coil protein golgin 45 interacts with GRASP55 and the GTP form of Rab 2, suggesting that GRASP55 and golgin 45 form a Rab 2 effector complex on medial-Golgi essential for normal protein transport and Golgi structure. ERK2 directly phosphorylates GRASP55, which is phosphorylated in mitotic cells, suggesting that mitogen-activated protein kinase kinase (MKK)/ERK pathway phosphorylates the Golgi during mitosis.

NCBI and Uniprot Product Information

• NCBI GI #: 319803020
• NCBI GeneID: 26003
• NCBI Accession #: NP_001188357.1
• NCBI GenBank Nucleotide #: NP_001188357.1
• UniProt Accession #: Q9H8Y8
• Molecular Weight: ~ 47kDa
• NCBI Official Full Name: Golgi reassembly-stacking protein 2 isoform 2
• NCBI Official Synonym Full Names: golgi reassembly stacking protein 2
• NCBI Official Symbol: GORASP2
• NCBI Official Synonym Symbols: p59; GRS2; GOLPH6; GRASP55
• NCBI Protein Information: Golgi reassembly-stacking protein 2
• UniProt Protein Name: Golgi reassembly-stacking protein 2
• UniProt Gene Name: GORASP2
• UniProt Synonym Gene Names: GOLPH6; GRS2; GOLPH6; GRASP55
• UniProt Entry Name: GORS2_HUMAN

NCBI Description

This gene encodes a member of the Golgi reassembly stacking protein family. These proteins may play a role in the stacking of Golgi cisternae and Golgi ribbon formation, as well as Golgi fragmentation during apoptosis or mitosis. The encoded protein also plays a role in the intracellular transport of transforming growth factor alpha and may function as a molecular chaperone. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

GRASP55: a golgi reassembly-stacking protein (GRASP) protein involved in the stacking of Golgi cisternae. May be involved in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as the TGF-alpha receptor. Oligomerization of its N-terminal GRASP domain regulates the formation of Golgi stacks, a process that is regulated by phosphorylation within the C-terminal Ser/Pro-rich domain. Forms a RAB2 effector complex with BLZF1 in the medial Golgi. Interacts with members of the p24 cargo receptors. Interacts with CNIH and the cytoplasmic domain of the TGF-alpha receptor, prior its transit in the trans-Golgi. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Vesicle

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: Golgi membrane; Golgi apparatus; membrane

Molecular Function: protein binding

Biological Process: organelle organization and biogenesis; mitotic cell cycle