Testing Data
(HEK293 overexpressing GRAP2 MBS624487 and probed with mock transfection in first lane),)
Goat anti-Human GRAP2 Polyclonal Antibody | anti-GRAP2 antibody
GRAP2 (GRB2 Related Adaptor Protein 2, GRB2 Like Protein, GADS, GRB2 Related Protein With Insert Domain, GRB2L, GRBLG, GrbX, Grf40, GRID, Growth Factor Receptor Binding Protein, GRPL, SH3-SH2-SH3 Adaptor Molecule)
Gene Names
GRAP2; P38; GADS; GRID; GRPL; GrbX; Mona; GRB2L; GRBLG; Grf40; GRAP-2
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by peptide affinity chromatography.
Synonyms
GRAP2; Polyclonal Antibody; GRAP2 (GRB2 Related Adaptor Protein 2; GRB2 Like Protein; GADS; GRB2 Related Protein With Insert Domain; GRB2L; GRBLG; GrbX; Grf40; GRID; Growth Factor Receptor Binding Protein; GRPL; SH3-SH2-SH3 Adaptor Molecule); Anti -GRAP2 (GRB2 Related Adaptor Protein 2; anti-GRAP2 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GRAP2. Species sequence homology: mouse, porcine.
Purity/Purification
Affinity Purified
Purified by peptide affinity chromatography.
Purified by peptide affinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Applicable Applications for anti-GRAP2 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Peptide ELISA Titer: 1:32,000
Western Blot: 1-3ug/ml detects ~38kD band observed lysates of cell line Jurkat (calculated MW of 37.9kD according to NP_004801.1). In transfected HEK293 transiently expressing GRAP2 a band of approx. 40kD is observed. This band is not observed in the non-transfected HEK293.
Dilution: Peptide ELISA Titer: 1:32,000
Western Blot: 1-3ug/ml detects ~38kD band observed lysates of cell line Jurkat (calculated MW of 37.9kD according to NP_004801.1). In transfected HEK293 transiently expressing GRAP2 a band of approx. 40kD is observed. This band is not observed in the non-transfected HEK293.
Immunogen
Synthetic peptide corresponding to C-GLFPANYVAPMTR, from the C Terminus of human GRAP2 (NP_004801.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(HEK293 overexpressing GRAP2 MBS624487 and probed with mock transfection in first lane),)
Testing Data
(HEK293 overexpressing GRAP2 MBS624487 and probed with mock transfection in first lane),)
Western Blot (WB)
(MBS624487 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(MBS624487 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Related Product Information for anti-GRAP2 antibody
This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist.
Product Categories/Family for anti-GRAP2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,909 Da
NCBI Official Full Name
GRB2-related adapter protein 2
NCBI Official Synonym Full Names
GRB2-related adaptor protein 2
NCBI Official Symbol
GRAP2
NCBI Official Synonym Symbols
P38; GADS; GRID; GRPL; GrbX; Mona; GRB2L; GRBLG; Grf40; GRAP-2
NCBI Protein Information
GRB2-related adapter protein 2; grf-40; GRB-2-like protein; OTTHUMP00000199126; OTTHUMP00000199127; adapter protein GRID; grf40 adapter protein; SH3-SH2-SH3 adapter Mona; SH3-SH2-SH3 adaptor molecule; growth factor receptor-binding protein; GRB2-related protein with insert domain; hematopoietic cell-associated adapter protein GrpL; hematopoietic cell-associated adaptor protein GRPL; growth factor receptor-bound protein 2-related adaptor protein 2
UniProt Protein Name
GRB2-related adapter protein 2
Protein Family
UniProt Gene Name
GRAP2
UniProt Synonym Gene Names
GADS; GRB2L; GRID
UniProt Entry Name
GRAP2_HUMAN
NCBI Description
This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq]
Uniprot Description
GADS: Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc. Belongs to the GRB2/sem-5/DRK family.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 22q13.2
Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol; endosome
Molecular Function: protein binding; SH3/SH2 adaptor activity
Biological Process: cell-cell signaling; positive regulation of signal transduction; T cell costimulation; Ras protein signal transduction; innate immune response; T cell receptor signaling pathway
Research Articles on GRAP2
Similar Products
Product Notes
The GRAP2 grap2 (Catalog #AAA624487) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The GRAP2 (GRB2 Related Adaptor Protein 2, GRB2 Like Protein, GADS, GRB2 Related Protein With Insert Domain, GRB2L, GRBLG, GrbX, Grf40, GRID, Growth Factor Receptor Binding Protein, GRPL, SH3-SH2-SH3 Adaptor Molecule) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GRAP2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Peptide ELISA Titer: 1:32,000 Western Blot: 1-3ug/ml detects ~38kD band observed lysates of cell line Jurkat (calculated MW of 37.9kD according to NP_004801.1). In transfected HEK293 transiently expressing GRAP2 a band of approx. 40kD is observed. This band is not observed in the non-transfected HEK293. Researchers should empirically determine the suitability of the GRAP2 grap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRAP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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