Goat anti-Human GPX4 Polyclonal Antibody | anti-GPX4 antibody

GPX4 (Glutathione Peroxidase 4, Phospholipid Hydroperoxidase, HGNC:4556, PHGPx, snGPx, sperm nucleus glutathione peroxidase)

Cat. Num. AAA623380

• Gene Names: GPX4; MCSP; PHGPx; snGPx; snPHGPx
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: GPX4; Polyclonal Antibody; GPX4 (Glutathione Peroxidase 4; Phospholipid Hydroperoxidase; HGNC:4556; PHGPx; snGPx; sperm nucleus glutathione peroxidase); Anti -GPX4 (Glutathione Peroxidase 4; anti-GPX4 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human GPX4.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-GPX4 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Peptide ELISA: 1:16,000.; Western Blot: 0.3-1ug/ml. A band is detected at 9kD in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1kDa according to NP_002076.2). Optimal dilutions to be determined by the researcher.
• Immunogen: A synthetic peptide from the C-terminus of aa-EEPLVIEKDLPHY.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Chemiluminescence

(MBS623380 (0.1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

(MBS623380 (0.3µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)

Product Categories/Family for anti-GPX4 antibody

Antibodies; Abs to Enzymes; Peroxidase

NCBI and Uniprot Product Information

• NCBI GI #: 33875003
• NCBI GeneID: 2879
• UniProt Accession #: Q6PJX4; P36969; Q6PI42
• Molecular Weight: 8,036 Da
• NCBI Official Full Name: GPX4 protein
• NCBI Official Synonym Full Names: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• NCBI Official Symbol: GPX4
• NCBI Official Synonym Symbols: MCSP; PHGPx; snGPx; snPHGPx
• NCBI Protein Information: phospholipid hydroperoxide glutathione peroxidase, mitochondrial; GPx-4; GSHPx-4; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase
• UniProt Protein Name: GPX4 protein
• Protein Family: Probable glutathione peroxidase
• UniProt Entry Name: Q6PJX4_HUMAN

NCBI Description

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. [provided by RefSeq]

Uniprot Description

GPX4: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Belongs to the glutathione peroxidase family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Mitochondrial; Lipid Metabolism - arachidonic acid; EC 1.11.1.12; Nuclear envelope; Other Amino Acids Metabolism - glutathione; Oxidoreductase

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: mitochondrion; cytosol; nucleus

Molecular Function: selenium binding; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity

Biological Process: glutathione metabolic process; lipoxygenase pathway; hydrogen peroxide catabolic process; phospholipid metabolic process; multicellular organismal development; regulation of inflammatory response; arachidonic acid metabolic process; response to oxidative stress; response to estradiol stimulus; aging