Goat GPX3 Polyclonal Antibody | anti-GPX3 antibody

Goat anti-GPX3 Antibody

Cat. Num. AAA420423

• Gene Names: GPX3; GPx-P; GSHPx-3; GSHPx-P
• Reactivity: Tested: Mouse, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: GPX3; Polyclonal Antibody; Goat anti-GPX3 Antibody; glutathione peroxidase 3 (plasma); GPx-P; GSHPx-3; GSHPx-P; extracellular glutathione peroxidase; plasma glutathione peroxidase 3; GPX3 antibody; glutathione peroxidase 3 (plasma) antibody; GPx-P antibody; GSHPx-3 antibody; GSHPx-P antibody; extracellular glutathione peroxidase antibody; plasma glutathione peroxidase 3 antibody; anti-GPX3 antibody

• Host: Goat
• Reactivity: Tested: Mouse, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: NQFGKQEPGENSE
• Sequence Length: 226

• Applicable Applications for anti-GPX3 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 1000.; Immunohistochemistry: Frozen sections of Porcin Kidney shows vesicular staining of the cytoplasm in PCT epithelial cells. Recommended concentration, 20ug/ml.; Western Blot: Approx 26kDa band observed in Mouse Kidney and Liver lysates (calculated MW of 27.7kDa according to Mouse NP_001077398.1 [isoform1], 25.4kDa according to Mouse NP_032187.2 [isoform 2] and 25.5kDa according to Human NP_002075.2). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence C-NQFGKQEPGENSE, from the internal region of the protein sequence according to NP_002075.2.
• Epitope: Internal region
• Note: This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunofluorescence (IF)

((20ug/ml) staining of PFA-perfused cryosection of Porcin Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of Zagreb, Croatia)

NCBI and Uniprot Product Information

• NCBI GI #: 6006001
• NCBI GeneID: 2878
• NCBI Accession #: NP_002075.2
• NCBI GenBank Nucleotide #: NM_002084.3
• Molecular Weight: 25,552 Da
• NCBI Official Full Name: glutathione peroxidase 3
• NCBI Official Synonym Full Names: glutathione peroxidase 3
• NCBI Official Symbol: GPX3
• NCBI Official Synonym Symbols: GPx-P; GSHPx-3; GSHPx-P
• NCBI Protein Information: glutathione peroxidase 3
• UniProt Protein Name: Glutathione peroxidase 3
• Protein Family: Probable glutathione peroxidase
• UniProt Gene Name: GPX3
• UniProt Synonym Gene Names: GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P
• UniProt Entry Name: GPX3_HUMAN

NCBI Description

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

Uniprot Description

GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family.

Protein type: EC 1.11.1.9; Oxidoreductase; Lipid Metabolism - arachidonic acid; Secreted; Other Amino Acids Metabolism - glutathione; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: extracellular region; extracellular space

Molecular Function: glutathione peroxidase activity; protein binding; selenium binding; transcription factor binding

Biological Process: hydrogen peroxide catabolic process; protein homotetramerization; response to lipid hydroperoxide; response to reactive oxygen species