Rabbit anti-Human GPRASP1 Polyclonal Antibody | anti-GPRASP1 antibody

GPRASP1, CT (GPRASP1, GASP, KIAA0443, G-protein coupled receptor-associated sorting protein 1) (FITC)

Cat. Num. AAA6304099

• Gene Names: GPRASP1; GASP; GASP1; GASP-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPRASP1; Polyclonal Antibody; CT (GPRASP1; GASP; KIAA0443; G-protein coupled receptor-associated sorting protein 1) (FITC); G-protein coupled receptor-associated sorting protein 1; anti-GPRASP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GPRASP1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GPRASP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1156-1184 amino acids from the C-terminal region of human GPRASP1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GPRASP1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GPRASP1 antibody detected the GPRASP1 protein (arrow).)

Immunohistochemistry (IHC)

(GPRASP1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPRASP1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GPRASP1 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GPRASP1 antibody

Antibodies; G Protein Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 296080706
• NCBI GeneID: 9737
• NCBI Accession #: NP_001171656.1; NP_001092881.1; NP_055525.3
• NCBI GenBank Nucleotide #: NM_001184727.1
• UniProt Accession #: Q5JY77; O43168; Q96LA1
• Molecular Weight: 156,865 Da
• NCBI Official Full Name: G-protein coupled receptor-associated sorting protein 1
• NCBI Official Synonym Full Names: G protein-coupled receptor associated sorting protein 1
• NCBI Official Symbol: GPRASP1
• NCBI Official Synonym Symbols: GASP; GASP1; GASP-1
• NCBI Protein Information: G-protein coupled receptor-associated sorting protein 1
• UniProt Protein Name: G-protein coupled receptor-associated sorting protein 1
• Protein Family: G-protein coupled receptor-associated sorting protein
• UniProt Gene Name: GPRASP1
• UniProt Synonym Gene Names: GASP; KIAA0443; GASP-1
• UniProt Entry Name: GASP1_HUMAN

NCBI Description

This gene encodes a member of the GPRASP (G protein-coupled receptor associated sorting protein) family. The protein may modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. It targets receptors for degradation in lysosomes. The receptors interacting with this sorting protein include D2 dopamine receptor (DRD2), delta opioid receptor (OPRD1), beta-2 adrenergic receptor (ADRB2), D4 dopamine receptor (DRD4) and cannabinoid 1 receptor (CB1R). Multiple alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, May 2010]

Uniprot Description

GPRASP1: May modulate lysosomal sorting and functional down- regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes. Belongs to the GPRASP family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: Xq22.1

Cellular Component: cytoplasm

Molecular Function: protein binding

Biological Process: endosome to lysosome transport