Rabbit anti-Human GPR83 Polyclonal Antibody | anti-GPR83 antibody

GPR83, NT (GPR83, GPR72, KIAA1540, Probable G-protein coupled receptor 83, G-protein coupled receptor 72) (Azide free) (HRP)

Cat. Num. AAA6304045

• Gene Names: GPR83; GIR; GPR72
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPR83; Polyclonal Antibody; NT (GPR83; GPR72; KIAA1540; Probable G-protein coupled receptor 83; G-protein coupled receptor 72) (Azide free) (HRP); G-protein coupled receptor 72; anti-GPR83 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GPR83 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GPR83 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GPR83.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GPR83 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GPR83 antibody detected the GPR83 protein (arrow).)

Related Product Information for anti-GPR83 antibody

GPR83 is an orphan receptor. Could be a neuropeptide Y receptor.

Product Categories/Family for anti-GPR83 antibody

Antibodies; G Protein Receptors

References

Hansen, W., et al. Genes Immun. 11(4):357-361(2010). Lamesch, P., et al. Genomics 89(3):307-315(2007). Hansen, W., et al. J. Immunol. 177(1):209-215(2006). Parker, R., et al. Biochim. Biophys. Acta 1491 (1-3), 369-375 (2000): De Moerlooze, L., et al. Cytogenet. Cell Genet. 90 (1-2), 146-150 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 223633971
• NCBI GeneID: 10888
• NCBI Accession #: NP_057624.3
• NCBI GenBank Nucleotide #: NM_016540.3
• UniProt Accession #: Q9NYM4; Q6NWR4; Q9P1Y8; B0M0K5
• Molecular Weight: 48,339 Da
• NCBI Official Full Name: probable G-protein coupled receptor 83
• NCBI Official Synonym Full Names: G protein-coupled receptor 83
• NCBI Official Symbol: GPR83
• NCBI Official Synonym Symbols: GIR; GPR72
• NCBI Protein Information: probable G-protein coupled receptor 83; G protein-coupled receptor 72; G-protein coupled receptor 72; glucocorticoid induced receptor
• UniProt Protein Name: Probable G-protein coupled receptor 83
• Protein Family: Probable G-protein coupled receptor
• UniProt Gene Name: GPR83
• UniProt Synonym Gene Names: GPR72; KIAA1540
• UniProt Entry Name: GPR83_HUMAN

Uniprot Description

GPR83: Orphan receptor. Could be a neuropeptide Y receptor. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: nonmotile primary cilium; plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; neuropeptide Y receptor activity

Biological Process: neuropeptide signaling pathway; response to glucocorticoid stimulus