Rabbit GPR44 / CRTH2 Polyclonal Antibody | anti-GPR44 antibody

Rabbit Polyclonal to Human GPR44 / CRTH2

Cat. Num. AAA243490

• Gene Names: PTGDR2; DP2; DL1R; CD294; CRTH2; GPR44
• Reactivity: Gorilla, Human, Monkey; Predicted Reactivity: Gibbon, Dog (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Immunocytochemistry
• Purity: Immunoaffinity Purified
• Synonyms: GPR44 / CRTH2; Polyclonal Antibody; Rabbit Polyclonal to Human GPR44 / CRTH2; Anti-GPR44 / CRTH2 Antibody (Extracellular Domain) IHC-plus; PTGDR2; CD294; CRTH2; CD294 antigen; DP2; DL1R; G protein-coupled receptor 44; G-protein coupled receptor 44; HCRTH2; DP2 prostaglandin receptor; GPR44; Prostaglandin D2 receptor 2; Human GPR44; anti-GPR44 antibody

• Host: Rabbit
• Reactivity: Gorilla, Human, Monkey; Predicted Reactivity: Gibbon, Dog (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human GPR44 / CRTH2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 395

• Applicable Applications for anti-GPR44 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC)
• Application Notes: ICC, IHC-P (16 - 32 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic 20 amino acid peptide from 3rd extracellular domain of human GPR44 / CRTH2. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%); Gibbon (95%); Panda, Dog (90%); Elephant, Bovine, Horse (85%).
• Immunogen Type: Synthetic peptide
• Immunogen: GPR44 / CRTH2 antibody was raised against synthetic 20 amino acid peptide from 3rd extracellular domain of human GPR44 / CRTH2. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%); Gibbon (95%); Panda, Dog (90%); Elephant, Bovine, Horse (85%); Rabbit, Pig, Guinea pig (80%).
• Antigen Modification: Extracellular Domain
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-GPR44 / CRTH2 antibody IHC of human nasal mucosa, eosinophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Transfection

(Anti-GPR44 / CRTH2 antibody immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with GPR44 / CRTH2.)

Transfection Control

(Anti-GPR44 / CRTH2 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)

Related Product Information for anti-GPR44 antibody

GPR44, also called CRTH2, is a Prostanoid Receptor that binds prostaglandin D2. PGD2 signals through CRTH2 to induce Th2, eosinophil, and basophil chemotaxis in a G-alpha(i) dependent manner. CRTH2 may play a role in mast cell-mediated immune responses such as those involved in allergic reactions. This receptor is upregulated in activated T helper-2 (Th2) cells, peripheral blood basophils, and eosinophils, but not in Th1 lineage cells. It may be a useful marker for Th2 and Tc2 cells.

Product Categories/Family for anti-GPR44 antibody

Family: GPCR; Subfamily: Prostanoid

NCBI and Uniprot Product Information

• NCBI GI #: 153791424
• NCBI GeneID: 11251
• NCBI Accession #: NP_004769.2
• NCBI GenBank Nucleotide #: NM_004778.2
• UniProt Accession #: Q9Y5Y4; O94765; Q4QRI6
• Molecular Weight: 43,268 Da
• NCBI Official Full Name: prostaglandin D2 receptor 2
• NCBI Official Synonym Full Names: prostaglandin D2 receptor 2
• NCBI Official Symbol: PTGDR2
• NCBI Official Synonym Symbols: DP2; DL1R; CD294; CRTH2; GPR44
• NCBI Protein Information: prostaglandin D2 receptor 2; G protein-coupled receptor 44; G-protein coupled receptor 44; putative G-protein coupled receptor 44; chemoattractant receptor-homologous molecule expressed on TH2 cells; chemoattractant receptor homologous molecule expressed on T helper type 2 cells
• UniProt Protein Name: Prostaglandin D2 receptor 2
• UniProt Gene Name: PTGDR2
• UniProt Synonym Gene Names: CRTH2; DL1R; GPR44
• UniProt Entry Name: PD2R2_HUMAN

NCBI Description

This gene encodes a G-protein-coupled receptor that is preferentially expressed in CD4+ effector T helper 2 (Th2) cells. This protein is a prostaglandin D2 receptor that mediates the pro-inflammatory chemotaxis of eosinophils, basophils, and Th2 lymphocytes generated during allergic inflammation. Single nucleotide polymorphisms in the 3' UTR of this gene have been associated with asthma susceptibility.[provided by RefSeq, Mar 2011]

Uniprot Description

GPR44: Receptor for prostaglandin D2 (PGD2). Coupled to the G(i)-protein. Receptor activation may result in pertussis toxin- sensitive decreases in cAMP levels and Ca(2+) mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, ADRBK1/GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12-q13.3

Cellular Component: neuron projection; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; neuropeptide binding; prostaglandin F receptor activity; prostaglandin J receptor activity; prostaglandin D receptor activity

Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; synaptic transmission; G-protein coupled receptor protein signaling pathway; neuropeptide signaling pathway; positive regulation of G-protein coupled receptor protein signaling pathway; calcium-mediated signaling; immune response; G-protein signaling, adenylate cyclase inhibiting pathway; chemotaxis