Rabbit CRTH2 Polyclonal Antibody | anti-GPR44 antibody

CRTH2 Antibody

Cat. Num. AAA150173

• Gene Names: PTGDR2; DP2; DL1R; CD294; CRTH2; GPR44
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: CRTH2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: CRTH2; Polyclonal Antibody; CRTH2 Antibody; DP2; DL1R; CD294; GPR44; Prostaglandin D2 receptor 2; Chemoattractant receptor-homologous molecule expressed on Th2 cells; G protein-coupled receptor 44; anti-GPR44 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two different isoforms of CRTH2 are known to exist; this antibody will detect both isoforms.
• Purity/Purification: CRTH2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 395

• Applicable Applications for anti-GPR44 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: CRTH2 antibody can be used for detection of CRTH2 by Western blot a 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL.; Optimal dilutions for each application to be determined by the researcher.
• Conjugate: Unconjugated
• Immunogen: CRTH2 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human CRTH2.; The immuogen is located within the first 50 amino acids of CRTH2.
• Buffer: CRTH2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Positive Control: Jurkat Cell Lysate-MBS151729; Human Heart Tissue Lysate-MBS151755; Human Heart Tissue Slide- MBS154198
• Preparation and Storage: CRTH2 antibody can be stored at 4°C for three months and -20°C , stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CRTH2 in Jurkat cell lysate with CRTH2 antibody at 1 μg/mL in (A) the absence and (B) presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of CRTH2 in human heart tissue with CRTH2 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of CRTH2 in Human Heart tissue with CRTH2 antibody at 20 μg/mL.)

Related Product Information for anti-GPR44 antibody

CRTH2 Antibody: The chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) is a recently identified receptor for the prostaglandin D2 (PGD2) in addition to the classic prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo.

NCBI and Uniprot Product Information

• NCBI GI #: 153791424
• NCBI GeneID: 11251
• NCBI Accession #: NP_004769
• NCBI GenBank Nucleotide #: NM_004778.2
• UniProt Accession #: Q9Y5Y4; O94765; Q4QRI6
• Molecular Weight: Predicted: 43, 55 kDa; Obderved: 60kDa
• NCBI Official Full Name: prostaglandin D2 receptor 2
• NCBI Official Synonym Full Names: prostaglandin D2 receptor 2
• NCBI Official Symbol: PTGDR2
• NCBI Official Synonym Symbols: DP2; DL1R; CD294; CRTH2; GPR44
• NCBI Protein Information: prostaglandin D2 receptor 2; G protein-coupled receptor 44; G-protein coupled receptor 44; putative G-protein coupled receptor 44; chemoattractant receptor-homologous molecule expressed on TH2 cells; chemoattractant receptor homologous molecule expressed on T helper type 2 cells
• UniProt Protein Name: Prostaglandin D2 receptor 2
• UniProt Gene Name: PTGDR2
• UniProt Synonym Gene Names: CRTH2; DL1R; GPR44
• UniProt Entry Name: PD2R2_HUMAN

NCBI Description

This gene encodes a G-protein-coupled receptor that is preferentially expressed in CD4+ effector T helper 2 (Th2) cells. This protein is a prostaglandin D2 receptor that mediates the pro-inflammatory chemotaxis of eosinophils, basophils, and Th2 lymphocytes generated during allergic inflammation. Single nucleotide polymorphisms in the 3' UTR of this gene have been associated with asthma susceptibility.[provided by RefSeq, Mar 2011]

Uniprot Description

GPR44: Receptor for prostaglandin D2 (PGD2). Coupled to the G(i)-protein. Receptor activation may result in pertussis toxin- sensitive decreases in cAMP levels and Ca(2+) mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, ADRBK1/GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 11q12-q13.3

Cellular Component: neuron projection; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; neuropeptide binding; prostaglandin F receptor activity; prostaglandin J receptor activity; prostaglandin D receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; synaptic transmission; G-protein signaling, coupled to cyclic nucleotide second messenger; neuropeptide signaling pathway; positive regulation of G-protein coupled receptor protein signaling pathway; calcium-mediated signaling; immune response; G-protein signaling, adenylate cyclase inhibiting pathway; chemotaxis