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Western Blot (WB) (Western blot (WB) analysis of RAB11FIP4 polyclonal antibody at 1:500 dilutionLaneA: The testis tissue lysate of RatLaneB: The testis tissue lysate of MouseLaneC: PC3 whole cell lysateLaneD: LOVO whole cell lysateLaneE: A549 whole cell lysate)

Rabbit GPR194 Polyclonal Antibody | anti-GPR194 antibody

GPR194 polyclonal antibody

Gene Names
RAB11FIP4; RAB11-FIP4
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
Synonyms
GPR194; Polyclonal Antibody; GPR194 polyclonal antibody; Rab11 family-interacting protein 4; FIP4-Rab11; Rab11-FIP4; Arfophilin-2; RAB11FIP4; ARFO2; KIAA1821; anti-GPR194 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
RAB11FIP4 polyclonal antibody detects endogenous levels of RAB11FIP4 protein.
Purity/Purification
The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.4
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GPR194 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500~1000
Immunogen
Synthetic peptide, corresponding to Human RAB11FIP4
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -25 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of RAB11FIP4 polyclonal antibody at 1:500 dilutionLaneA: The testis tissue lysate of RatLaneB: The testis tissue lysate of MouseLaneC: PC3 whole cell lysateLaneD: LOVO whole cell lysateLaneE: A549 whole cell lysate)

Western Blot (WB) (Western blot (WB) analysis of RAB11FIP4 polyclonal antibody at 1:500 dilutionLaneA: The testis tissue lysate of RatLaneB: The testis tissue lysate of MouseLaneC: PC3 whole cell lysateLaneD: LOVO whole cell lysateLaneE: A549 whole cell lysate)
Related Product Information for anti-GPR194 antibody
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas9) system is an adaptive immune response defense mechanism used by archea and bacteria for the degradation of foreign genetic material. This mechanism can be repurposed for other functions, including genomic engineering for mammalian systems, such as gene knockout (KO) and gene activation. CRISPR Activation Plasmid products enable the identification and upregulation of specific genes by utilizing a D10A and N863A deactivated Cas9 (dCas9) nuclease fused to a VP64 activation domain, in conjunction with sgRNA (MS2), a target-specific sgRNA engineered to bind the MS2-P65-HSF1 fusion protein. This synergistic activation mediator (SAM) transcription activation system provides a robust system to maximize the activation of endogenous gene expression.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60,771 Da
NCBI Official Full Name
rab11 family-interacting protein 4 isoform 1
NCBI Official Synonym Full Names
RAB11 family interacting protein 4 (class II)
NCBI Official Symbol
RAB11FIP4
NCBI Official Synonym Symbols
RAB11-FIP4
NCBI Protein Information
rab11 family-interacting protein 4
UniProt Protein Name
Rab11 family-interacting protein 4
UniProt Gene Name
RAB11FIP4
UniProt Synonym Gene Names
ARFO2; KIAA1821; FIP4-Rab11; Rab11-FIP4
UniProt Entry Name
RFIP4_HUMAN

NCBI Description

Proteins of the large Rab GTPase family (see RAB1A; MIM 179508) have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP4 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001 [PubMed 11495908]).[supplied by OMIM, Apr 2008]

Uniprot Description

RAB11FIP4: Acts as a regulator of endocytic traffic by participating in membrane delivery. Required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis. In case of infection by HCMV (human cytomegalovirus), may participate in egress of the virus out of nucleus; this function is independent of ARF6. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cleavage furrow; endocytic vesicle; endosome; extracellular space; microtubule organizing center; midbody; recycling endosome membrane; spindle

Molecular Function: ADP-ribosylation factor binding; calcium ion binding; protein binding; protein homodimerization activity; Rab GTPase binding

Biological Process: cytokinesis; transport; viral reproduction

Research Articles on GPR194

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Product Notes

The GPR194 rab11fip4 (Catalog #AAA3014884) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR194 polyclonal antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GPR194 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500~1000. Researchers should empirically determine the suitability of the GPR194 rab11fip4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR194, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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