Rabbit GPR189 Polyclonal Antibody | anti-GPR189 antibody

GPR189 polyclonal antibody

Cat. Num. AAA3014879

• Gene Names: APOBEC4; C1orf169
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot
• Purity: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Synonyms: GPR189; Polyclonal Antibody; GPR189 polyclonal antibody; Putative C->U-editing enzyme APOBEC-4; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 4; APOBEC4; C1orf169; anti-GPR189 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: APOBEC4 polyclonal antibody detects endogenous levels of APOBEC4 protein.
• Purity/Purification: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GPR189 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1000
• Immunogen: Synthetic peptide, corresponding to Human APOBEC4
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -25 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of APOBEC4 polyclonal antibody at 1:500 dilutionLaneA: The testis tissue lysate of RatLaneB: The testis tissue lysate of MouseLaneC: MCF-7 whole cell lysateLaneD: PC3 whole cell lysate)

Related Product Information for anti-GPR189 antibody

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas9) system is an adaptive immune response defense mechanism used by archea and bacteria for the degradation of foreign genetic material. This mechanism can be repurposed for other functions, including genomic engineering for mammalian systems, such as gene knockout (KO) and gene activation. CRISPR Activation Plasmid products enable the identification and upregulation of specific genes by utilizing a D10A and N863A deactivated Cas9 (dCas9) nuclease fused to a VP64 activation domain, in conjunction with sgRNA (MS2), a target-specific sgRNA engineered to bind the MS2-P65-HSF1 fusion protein. This synergistic activation mediator (SAM) transcription activation system provides a robust system to maximize the activation of endogenous gene expression.

NCBI and Uniprot Product Information

• NCBI GI #: 44888831
• NCBI GeneID: 403314
• NCBI Accession #: NP_982279.1
• NCBI GenBank Nucleotide #: NM_203454.2
• UniProt Accession #: Q8WW27; Q8N7F6
• Molecular Weight: 41,581 Da
• NCBI Official Full Name: putative C->U-editing enzyme APOBEC-4
• NCBI Official Synonym Full Names: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 4 (putative)
• NCBI Official Symbol: APOBEC4
• NCBI Official Synonym Symbols: C1orf169
• NCBI Protein Information: putative C->U-editing enzyme APOBEC-4; apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 4
• UniProt Protein Name: Putative C->U-editing enzyme APOBEC-4
• UniProt Gene Name: APOBEC4
• UniProt Synonym Gene Names: C1orf169
• UniProt Entry Name: ABEC4_HUMAN

NCBI Description

This gene encodes a member of the AID/APOBEC family of polynucleotide (deoxy)cytidine deaminases, which convert cytidine to uridine. Other AID/APOBEC family members are involved in mRNA editing, somatic hypermutation and recombination of immunoglobulin genes, and innate immunity to retroviral infection. [provided by RefSeq, Jul 2008]

Uniprot Description

APOBEC4: Putative C to U editing enzyme whose physiological substrate is not yet known. Belongs to the cytidine and deoxycytidylate deaminase family.

Protein type: EC 3.5.4.-; Hydrolase

Chromosomal Location of Human Ortholog: 1q25.3

Molecular Function: zinc ion binding; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines

Biological Process: mRNA processing