Rabbit anti-Human, Mouse GPR185 Polyclonal Antibody | anti-GPR185 antibody

GPR185 polyclonal antibody

Cat. Num. AAA3014875

• Gene Names: PPM1L; PP2CE; PPM1-LIKE; PP2C-epsilon
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Synonyms: GPR185; Polyclonal Antibody; GPR185 polyclonal antibody; Protein phosphatase 1L; Protein phosphatase 1-like; Protein phosphatase 2C isoform epsilon; PP2C-epsilon; PPM1L; PP2CE; anti-GPR185 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PPM1L polyclonal antibody detects endogenous levels of PPM1L protein.
• Purity/Purification: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GPR185 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1000
• Immunogen: Synthetic peptide, corresponding to Human PPM1L
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -25 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of PPM1L polyclonal antibody at 1:500 dilutionLaneA: Aml-12 whole cell lysateLaneB: H1792 whole cell lysateLaneC: COS-7 whole cell lysate)

Related Product Information for anti-GPR185 antibody

Protein phosphatase 1L, acts as a suppressor of the SAPK signaling pathways by associating with and dephosphorylating MAP3K7/TAK1 and MAP3K5, and by attenuating the association between MAP3K7/TAK1 and MAP2K4 or MAP2K6.

NCBI and Uniprot Product Information

• NCBI GI #: 63003905
• NCBI GeneID: 151742
• NCBI Accession #: NP_640338.2
• NCBI GenBank Nucleotide #: NM_139245.2
• UniProt Accession #: Q5SGD2; Q2M3J2; Q96NM7
• Molecular Weight: 41,053 Da
• NCBI Official Full Name: protein phosphatase 1L
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1L
• NCBI Official Symbol: PPM1L
• NCBI Official Synonym Symbols: PP2CE; PPM1-LIKE; PP2C-epsilon
• NCBI Protein Information: protein phosphatase 1L; PP2C epsilon; Protein phosphatase 2C epsilon isoform; protein phosphatase 2C isoform epsilon
• UniProt Protein Name: Protein phosphatase 1L
• UniProt Gene Name: PPM1L
• UniProt Synonym Gene Names: PP2CE; PP2C-epsilon
• UniProt Entry Name: PPM1L_HUMAN

NCBI Description

PPM1L, or PP2CE, belongs to the PP2C group of serine/threonine phosphatases, which are distinguished from other phosphatases by their structure, absolute requirement for Mg(2+) or Mn(2+), and insensitivity to okadaic acid. PP2Cs regulate stress-activated protein kinase (SAPK; see MIM 601158) signaling cascades that respond to extracellular stimuli (Jin et al., 2004 [PubMed 15560375]).[supplied by OMIM, Apr 2008]

Uniprot Description

PPM1L: Acts as a suppressor of the SAPK signaling pathways by associating with and dephosphorylating MAP3K7/TAK1 and MAP3K5, and by attenuating the association between MAP3K7/TAK1 and MAP2K4 or MAP2K6. Belongs to the PP2C family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16

Chromosomal Location of Human Ortholog: 3q26.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: metal ion binding; protein serine/threonine phosphatase activity

Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; transmembrane receptor protein serine/threonine kinase signaling pathway; MAPKKK cascade; protein amino acid dephosphorylation