Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using GPR177 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit GPR177 Polyclonal Antibody | anti-GPR177 antibody
GPR177 Antibody
Gene Names
WLS; EVI; MRP; GPR177; mig-14; C1orf139
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
GPR177; Polyclonal Antibody; GPR177 Antibody; C1orf139; DKFZp686I0788; Evenness interrupted; Drosophila; homolg of; EVI; FLJ23091; G protein coupled receptor 177; GPR 177; Integral membrane protein GPR177; MGC131760; MGC14878; mig-14; MRP; Protein evenness interrupted homolog; Protein wntless homolog; Putative NF-kappa-B-activating protein 373; Putative NFkB activating protein 373; WLS; WLS_HUMAN; wntless homolog (Drosophila); Wntless homolog; wntless Wnt ligand secretion mediator; Wntless; homolog of; anti-GPR177 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Clonality
Polyclonal
Isotype
IgG
Specificity
GPR177 Antibody detects endogenous levels of GPR177.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
543
Applicable Applications for anti-GPR177 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human GPR177.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Endoplasmic Reticulum; Endosome; Golgi Apparatus; Plasma Membrane
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using GPR177 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using GPR177 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-GPR177 antibody
Description: Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity).
Subunit Structure: Interacts with WNT3A. Interacts with WNT1, WNT3 and WNT5A (By similarity).
Similarity: Belongs to the wntless family.
Subunit Structure: Interacts with WNT3A. Interacts with WNT1, WNT3 and WNT5A (By similarity).
Similarity: Belongs to the wntless family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 62 kDa
Predicted: 63 kDa
Predicted: 63 kDa
NCBI Official Full Name
protein wntless homolog isoform 2
NCBI Official Synonym Full Names
Wnt ligand secretion mediator
NCBI Official Symbol
WLS
NCBI Official Synonym Symbols
EVI; MRP; GPR177; mig-14; C1orf139
NCBI Protein Information
protein wntless homolog
UniProt Protein Name
Protein wntless homolog
UniProt Gene Name
WLS
UniProt Synonym Gene Names
C1orf139; GPR177; EVI
UniProt Entry Name
WLS_HUMAN
Uniprot Description
GPR177: Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development. Belongs to the wntless family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p31.3
Cellular Component: dendrite cytoplasm; Golgi membrane; cytoplasmic vesicle membrane; early endosome membrane; integral to membrane; plasma membrane
Molecular Function: Wnt-protein binding; signal transducer activity; protein binding; mu-type opioid receptor binding
Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; Wnt receptor signaling pathway; mesoderm formation; anterior/posterior axis specification
Research Articles on GPR177
Similar Products
Product Notes
The GPR177 wls (Catalog #AAA9611802) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR177 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's GPR177 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the GPR177 wls for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR177, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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