Rabbit GPR174 Polyclonal Antibody | anti-GPR174 antibody

GPR174 polyclonal antibody

Cat. Num. AAA3014864

• Gene Names: PNPT1; OLD35; DFNB70; PNPASE; old-35; COXPD13
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Synonyms: GPR174; Polyclonal Antibody; GPR174 polyclonal antibody; Polyribonucleotide nucleotidyltransferase 1; mitochondrial; 3'-5' RNA exonuclease OLD35; PNPase old-35; Polynucleotide phosphorylase 1; PNPase 1; Polynucleotide phosphorylase-like protein; PNPT1; PNPASE; anti-GPR174 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PNPT1 polyclonal antibody detects endogenous levels of PNPT1 protein.
• Purity/Purification: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GPR174 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1000
• Immunogen: Synthetic peptide, corresponding to Human PNPT1
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -24 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of PNPT1 polyclonal antibody at 1:500 dilutionLaneA: PC12 whole cell lysateLaneB: MEF whole cell lysateLaneC: A549 whole cell lysateLaneD: L02 whole cell lysateLaneE: HCT116 whole cell lysate)

Related Product Information for anti-GPR174 antibody

Mitochondrial polyribonucleotide nucleotidyltransferase, also designated 3'-5' RNA exonuclease, OLD35, PNPase or PNPT1, is an evolutionaly conserved protein in which the mouse protein shares 90% identity with the human version. PNPase participates in mRNA degradation and hydrolyzes single-stranded ribonucleotides in the 3' to 5' direction. PNPase forms homotrimers and is upregulated in response to interferon-beta induction. The N-terminus of PNPase contains a putative mitochondrial targeting sequence; mutation analysis confirms that N-terminal sequences of PNPase target the protein to the mitochondria. Endogenous PNPase also co-localizes with a mitochondrial marker protein in HeLa cells.

NCBI and Uniprot Product Information

• NCBI GI #: 188528628
• NCBI GeneID: 87178
• NCBI Accession #: NP_149100.2
• NCBI GenBank Nucleotide #: NM_033109.4
• UniProt Accession #: Q8TCS8; Q53SU0; Q68CN1; Q7Z7D1; Q8IWX1; Q96T05; Q9BRU3; Q9BVX0
• Molecular Weight: 85,951 Da
• NCBI Official Full Name: polyribonucleotide nucleotidyltransferase 1, mitochondrial
• NCBI Official Synonym Full Names: polyribonucleotide nucleotidyltransferase 1
• NCBI Official Symbol: PNPT1
• NCBI Official Synonym Symbols: OLD35; DFNB70; PNPASE; old-35; COXPD13
• NCBI Protein Information: polyribonucleotide nucleotidyltransferase 1, mitochondrial; PNPase 1; PNPase old-35; 3'-5' RNA exonuclease OLD35; polynucleotide phosphorylase 1; polynucleotide phosphorylase-like protein
• UniProt Protein Name: Polyribonucleotide nucleotidyltransferase 1, mitochondrial
• Protein Family: Probable G-protein coupled receptor
• UniProt Gene Name: PNPT1
• UniProt Synonym Gene Names: PNPASE; PNPase 1
• UniProt Entry Name: PNPT1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7. [provided by RefSeq, Dec 2012]

Uniprot Description

PNPT1: RNA-binding protein implicated in numerous RNA metabolic processes. Hydrolyzes single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c- myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA. Belongs to the polyribonucleotide nucleotidyltransferase family.

Protein type: Mitochondrial; Nucleotide Metabolism - purine; EC 2.7.7.8; Nucleotide Metabolism - pyrimidine; Transferase; RNA-binding

Chromosomal Location of Human Ortholog: 2p15

Cellular Component: membrane; mitochondrion; cytoplasm; mitochondrial intermembrane space

Molecular Function: 3'-5'-exoribonuclease activity; protein binding; miRNA binding; poly(U) binding; polyribonucleotide nucleotidyltransferase activity; poly(rG) binding

Biological Process: regulation of cellular respiration; RNA polyadenylation; RNA catabolic process; mRNA catabolic process; protein homooligomerization; negative regulation of growth

Disease: Deafness, Autosomal Recessive 70; Combined Oxidative Phosphorylation Deficiency 13