Rabbit GPR146 Polyclonal Antibody | anti-GPR146 antibody

GPR146 polyclonal antibody

Cat. Num. AAA3014836

• Gene Names: MSX2; FPP; MSH; PFM; CRS2; HOX8; PFM1
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Synonyms: GPR146; Polyclonal Antibody; GPR146 polyclonal antibody; Homeobox protein MSX-2; Homeobox protein Hox-8; MSX2; HOX8; anti-GPR146 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: MSX2 polyclonal antibody detects endogenous levels of MSX2 protein.
• Purity/Purification: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GPR146 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1000
• Immunogen: Synthetic peptide, corresponding to Human MSX2
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of MSX2 polyclonal antibody at 1:500 dilutionLaneA: A549 whole cell lysateLaneB: HEK293T whole cell lysateLaneC: MEF whole cell lysateLaneD: H9C2 whole cell lysate)

Related Product Information for anti-GPR146 antibody

Msx homeobox genes encode for transcription factors that control morphogenesis and are expressed at sites of epithelial-mesenchymal interaction during embryogenesis, such as the Tooth. Two of these genes, Msx-1 and Msx-2, are key factors for the development of tooth and craniofacial skeleton. Msx-1 also down-regulates a master gene of skeletal cells differentiation. Msx-1 and Msx-2 contribute to the initial patterning of dentition as well as playing a pivotal role in terminal cell differentiation. In addition, Msx-1 and Msx-2 are expressed in the epidermis, hair follicles and fibroblasts of the developing fetal skin. In adult skin, Msx-1 and Msx-2 expression is confined to epithelially derived structures. Msx-2 is detected as a diffuse cytoplasmic signal in fetal epidermis and portions of the hair follicle and dermis, but is localized to the nucleus in the adult epidermis. Msx-1 and Msx-2 are also expressed during critical developmental stages of neural tube and neuralcrest, suggesting that these genes play an important role in organogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 27886557
• NCBI GeneID: 4488
• NCBI Accession #: NP_002440.2
• NCBI GenBank Nucleotide #: NM_002449.4
• UniProt Accession #: P35548; Q53XM4; Q9UD60; D3DQN1
• Molecular Weight: 28,897 Da
• NCBI Official Full Name: homeobox protein MSX-2
• NCBI Official Synonym Full Names: msh homeobox 2
• NCBI Official Symbol: MSX2
• NCBI Official Synonym Symbols: FPP; MSH; PFM; CRS2; HOX8; PFM1
• NCBI Protein Information: homeobox protein MSX-2; msh homeo box 2; homeobox protein Hox-8; msh homeobox homolog 2
• UniProt Protein Name: Homeobox protein MSX-2
• Protein Family: Probable G-protein coupled receptor
• UniProt Gene Name: MSX2
• UniProt Synonym Gene Names: HOX8
• UniProt Entry Name: MSX2_HUMAN

NCBI Description

This gene encodes a member of the muscle segment homeobox gene family. The encoded protein is a transcriptional repressor whose normal activity may establish a balance between survival and apoptosis of neural crest-derived cells required for proper craniofacial morphogenesis. The encoded protein may also have a role in promoting cell growth under certain conditions and may be an important target for the RAS signaling pathways. Mutations in this gene are associated with parietal foramina 1 and craniosynostosis type 2. [provided by RefSeq, Jul 2008]

Uniprot Description

MSX2: Acts as a transcriptional regulator in bone development. Represses the ALPL promoter activity and antogonizes the stimulatory effect of DLX5 on ALPL expression during osteoblast differentiation. Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE). Binds to the homeodomain-response element of the ALPL promoter. Defects in MSX2 are the cause of parietal foramina 1 (PFM1); also known as foramina parietalia permagna (FPP). PFM1 is an autosomal dominant disease characterized by oval defects of the parietal bones caused by deficient ossification around the parietal notch, which is normally obliterated during the fifth fetal month. Defects in MSX2 are the cause of parietal foramina with cleidocranial dysplasia (PFMCCD); also known as cleidocranial dysplasia with parietal foramina. PFMCCD combines skull defects in the form of enlarged parietal foramina and deficient ossification of the clavicles. Defects in MSX2 are the cause of craniosynostosis type 2 (CRS2); also known as craniosynostosis Boston-type (CSB). CRS2 is an autosomal dominant disorder characterized by the premature fusion of calvarial sutures. The craniosynostosis phenotype is either fronto-orbital recession, or frontal bossing, or turribrachycephaly, or cloverleaf skull. Associated features include severe headache, high incidence of visual problems (myopia or hyperopia), and short first metatarsals. Intelligence is normal. Belongs to the Msh homeobox family.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: transcription factor complex; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription cofactor activity; transcription factor binding

Biological Process: embryonic forelimb morphogenesis; transcription from RNA polymerase II promoter; negative regulation of keratinocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; chondrocyte development; negative regulation of fat cell differentiation; positive regulation of catagen; embryonic hindlimb morphogenesis; osteoblast development; osteoblast differentiation; anterior/posterior pattern formation; negative regulation of cell proliferation; positive regulation of osteoblast differentiation; inhibition of CREB transcription factor; negative regulation of transcription, DNA-dependent; positive regulation of BMP signaling pathway; wound healing, spreading of epidermal cells; negative regulation of apoptosis

Disease: Parietal Foramina; Parietal Foramina With Cleidocranial Dysplasia; Craniosynostosis 2