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Western Blot (WB) (Western blot analysis GPR105 using HT29 whole cell lysates)

Rabbit GPR105 Polyclonal Antibody | anti-GPR105 antibody

GPR105 Antibody

Gene Names
P2RY14; P2Y14; BPR105; GPR105
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GPR105; Polyclonal Antibody; GPR105 Antibody; A330108O13Rik; G protein coupled receptor for UDP-glucose; G protein-coupled receptor 105; G protein-coupled receptor VTR 15-20; G-protein coupled receptor 105; KIAA0001; P2RY14; P2Y purinoceptor 14; P2Y(14) receptor; P2Y14; P2Y14 receptor; P2Y14_HUMAN; Purinergic receptor P2Y G protein coupled 14; Similar to TYPE-1 Angiotensin II receptor; UDP-glucose receptor; anti-GPR105 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
GPR105 antibody detects endogenous levels of total GPR105
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
338
Applicable Applications for anti-GPR105 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human GPR105
Subcellular Location
Cell Membrane
Tissue Specificity
Highest expression in the placenta, adipose tissue, stomach and intestine, intermediate levels in the brain, spleen, lung and heart, lowest levels in the kidney.
Predicted Cross Reactivity
Pig, Rabbit, Dog
Similarity
Pig (83%), Rabbit (83%), Dog (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis GPR105 using HT29 whole cell lysates)

Western Blot (WB) (Western blot analysis GPR105 using HT29 whole cell lysates)

Immunofluorescene (IF)

(MBS9608979 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Immunofluorescene (IF) (MBS9608979 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-GPR105 antibody
Function: Receptor for UDP-glucose and other UDP-sugar coupled to G-proteins. Not activated by ATP, ADP, UTP or ATP.
Similarity: Belongs to the G-protein coupled receptor 1 family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 38 kDa
Predicted: 39 kDa
NCBI Official Full Name
P2Y purinoceptor 14
NCBI Official Synonym Full Names
purinergic receptor P2Y14
NCBI Official Symbol
P2RY14
NCBI Official Synonym Symbols
P2Y14; BPR105; GPR105
NCBI Protein Information
P2Y purinoceptor 14
UniProt Protein Name
P2Y purinoceptor 14
UniProt Gene Name
P2RY14
UniProt Synonym Gene Names
GPR105; KIAA0001; P2Y14

NCBI Description

The product of this gene belongs to the family of G-protein coupled receptors, which contains several receptor subtypes with different pharmacological selectivity for various adenosine and uridine nucleotides. This receptor is a P2Y purinergic receptor for UDP-glucose and other UDP-sugars coupled to G-proteins. It has been implicated in extending the known immune system functions of P2Y receptors by participating in the regulation of the stem cell compartment, and it may also play a role in neuroimmune function. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for UDP-glucose and other UDP-sugar coupled to G-proteins. Not activated by ATP, ADP, UTP or ATP.

Research Articles on GPR105

Similar Products

Product Notes

The GPR105 p2ry14 (Catalog #AAA9608979) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR105 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GPR105 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000-3000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the GPR105 p2ry14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR105, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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