Rabbit anti-Human, Mouse GPHN Polyclonal Antibody | anti-GPHN antibody

GPHN, ID (GPHN, GPH, KIAA1385, Gephyrin, Molybdopterin adenylyltransferase, Domain G, Molybdopterin molybdenumtransferase, Domain E) (FITC)

Cat. Num. AAA6303538

• Gene Names: GPHN; GPH; GEPH; HKPX1; GPHRYN; MOCODC
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPHN; Polyclonal Antibody; ID (GPHN; GPH; KIAA1385; Gephyrin; Molybdopterin adenylyltransferase; Domain G; Molybdopterin molybdenumtransferase; Domain E) (FITC); Domain E; anti-GPHN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GPHN antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GPHN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444~474 amino acids from the Central region of human GPHN.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GPHN Antibody (Center) western blot analysis in MCF-7,Hela,NCI-H460,HepG2 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the GPHN antibody detected the GPHN protein (arrow).)

Immunohistochemistry (IHC)

(GPHN Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPHN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GPHN Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GPHN antibody

GPHN encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency.

Product Categories/Family for anti-GPHN antibody

Antibodies; KIAA; orf

References

Tabakoff, B., et al. BMC Biol. 7, 70 (2009). Fritschy, J.M., et al. Trends Neurosci. 31(5):257-264(2008). Inada, T., et al. Pharmacogenet. Genomics 18(4):317-323(2008). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Ehrensperger, M.V., et al. Biophys. J. 92(10):3706-3718(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 8926308
• NCBI GeneID: 10243
• UniProt Accession #: Q9NQX3; Q96KU4; Q9H4E9; Q9P2G2
• Molecular Weight: 83,448 Da
• NCBI Official Full Name: gephyrin
• NCBI Official Synonym Full Names: gephyrin
• NCBI Official Symbol: GPHN
• NCBI Official Synonym Symbols: GPH; GEPH; HKPX1; GPHRYN; MOCODC
• NCBI Protein Information: gephyrin
• UniProt Protein Name: Gephyrin
• Protein Family: Gephyrin
• UniProt Gene Name: GPHN
• UniProt Synonym Gene Names: MPT adenylyltransferase; MPT Mo-transferase

NCBI Description

This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq, Jul 2008]

Uniprot Description

Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.